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rdf:type |
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lifeskim:mentions |
umls-concept:C0019564,
umls-concept:C0021701,
umls-concept:C0023516,
umls-concept:C0027882,
umls-concept:C0039194,
umls-concept:C0064830,
umls-concept:C0079091,
umls-concept:C1167622,
umls-concept:C1415843,
umls-concept:C1704675,
umls-concept:C1880022
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pubmed:issue |
3
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pubmed:dateCreated |
2000-4-19
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pubmed:abstractText |
Intercellular adhesion molecule-5 (ICAM-5, telencephalin) is a member of the immunoglobulin superfamily expressed on telencephalic neurons, and serves as a ligand for the leukocyte integrin CD11 a/CD18. We studied here the binding site in ICAM-5 for CD11a/CD18. Protein constructs containing the first immunoglobulin domain of ICAM-5 were able to support CD11a/CD18 interaction, while deletion of the first domain abolished binding. Monoclonal antibodies reacting with the first domain of ICAM-5 also completely blocked the interaction. The soluble first domain of ICAM-5 inhibited the binding of T cells to immobilized ICAM-5 at concentrations of 50 nM and higher. Interestingly, the sixth domain of ICAM-5 was also able to support leukocyte binding, but this binding activity may not involve leukocyte integrins. To test the involvement of ICAM-5 in leukocyte-neuron interactions, an assay using human T cells binding to rat hippocampal neurons was established. This binding was blocked by monoclonal antibodies against CD11a/CD18 and ICAM-5. Thus ICAM-5 may act as a major adhesion molecule for leukocyte binding to neurons in the central nervous system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD18,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/ICAM5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Function-Associated...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neural Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
30
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
810-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10741396-Animals,
pubmed-meshheading:10741396-Antibodies, Monoclonal,
pubmed-meshheading:10741396-Antigens, CD18,
pubmed-meshheading:10741396-Binding Sites,
pubmed-meshheading:10741396-Cell Adhesion Molecules,
pubmed-meshheading:10741396-Cells, Cultured,
pubmed-meshheading:10741396-Hippocampus,
pubmed-meshheading:10741396-Humans,
pubmed-meshheading:10741396-Lymphocyte Function-Associated Antigen-1,
pubmed-meshheading:10741396-Membrane Glycoproteins,
pubmed-meshheading:10741396-Nerve Tissue Proteins,
pubmed-meshheading:10741396-Neural Cell Adhesion Molecules,
pubmed-meshheading:10741396-Neurons,
pubmed-meshheading:10741396-Protein Structure, Tertiary,
pubmed-meshheading:10741396-Rats,
pubmed-meshheading:10741396-Recombinant Fusion Proteins,
pubmed-meshheading:10741396-T-Lymphocytes
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pubmed:year |
2000
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pubmed:articleTitle |
Binding of T lymphocytes to hippocampal neurons through ICAM-5 (telencephalin) and characterization of its interaction with the leukocyte integrin CD11a/CD18.
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pubmed:affiliation |
Department of Biosciences, University of Helskinki, Finland.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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