Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
|
pubmed:dateCreated |
1979-6-26
|
pubmed:abstractText |
Bacteriophage vectors derived from lambda plac5 have been constructed. Their genomes have one EcoRI restriction site which is located at the very beginning of the lac Z gene. The major part of this gene was deleted by an in vivo intramolecular recombination. These vectors allow the fusion of a gene or an operon with the beginning of the lac Z gene, placing them under the control of the lac promoter, which carries the UV5 mutation. Some of these vectors (lambda Y) also include the lac Y gene and it too is under the control of the lac promoter. The lambda YEQS, which carries the Qam73 and Sam7 mutations, as safety mutations, has been certified as a B2 (EK2) vector by the French control commission "recombinaison génétique in vitro".
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Feb
|
pubmed:issn |
0026-8925
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
26
|
pubmed:volume |
170
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
171-8
|
pubmed:dateRevised |
2000-12-18
|
pubmed:meshHeading |
pubmed-meshheading:107393-Coliphages,
pubmed-meshheading:107393-DNA, Recombinant,
pubmed-meshheading:107393-DNA, Viral,
pubmed-meshheading:107393-Escherichia coli,
pubmed-meshheading:107393-Genes, Viral,
pubmed-meshheading:107393-Microscopy, Electron,
pubmed-meshheading:107393-Nucleic Acid Conformation,
pubmed-meshheading:107393-Radiation Tolerance,
pubmed-meshheading:107393-Ultraviolet Rays,
pubmed-meshheading:107393-beta-Galactosidase
|
pubmed:year |
1979
|
pubmed:articleTitle |
Bacteriophage lambda-E. coli K12 vector-host system for gene cloning and expression under lactose promoter control. II. DNA fragment insertion at the vicinity of the lac UV5 promoter.
|
pubmed:publicationType |
Journal Article
|