Linked Life Data

10737963

Source:http://linkedlifedata.com/resource/pubmed/id/10737963

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-6-26
pubmed:abstractText
In most species, transcription is essentially silent during the first mitotic cell cycles that follow fertilization. This means that the regulation of gene expression in early embryos heavily relies on the translational activation or inactivation of maternal mRNAs. In mammals, the mechanisms that control the translation of maternal mRNAs have been mainly studied in the mouse when maternal to zygotic transition occurs after the first mitotic division. In other mammalian species, however, this transition occurs later after several cell cycles, and little is known concerning the regulation of maternal information during this period. To address this question, we have used rabbit pre-implantation embryos to analyze the translational activation and stability of two maternal mRNAs, mm 41 and mm61. During the cleavage period, these mRNAs exhibit distinct kinetics for both their translational activation and degradation. In addition, these mRNAs both undergo cytoplasmic polyadenylation but with different efficiencies. This polyadenylation was functionally correlated with the translational activation of these mRNAs; inhibiting polyadenylation prevented translational activation. The differential efficiency of cytoplasmic polyadenylation, driven by cis-elements in the 3' untranslated region of these mRNAs, was also observed in Xenopus laevis embryos, which emphasizes the high conservation of this mechanism between species.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1040-452X
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
12-25
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Differential regulation of the translation and the stability of two maternal transcripts in preimplantation rabbit embryos.
pubmed:affiliation
Laboratoire de Biologie du Développement, INRA, Jouy en Josas, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't