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pubmed-article:10737323pubmed:abstractTextAlpha2-3 and alpha2-6 sialyl linkage types of sialyllactoses and sialyl-N-acetyllactosamines were analyzed by post-source decay (PSD) fragmentation method using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. A new matrix of norharmane was suited for the MALDI-TOF measurements of sialyl oligosaccharides. The fragment ions B1 produced by the cleavage of alpha2-3 sialyl linkages indicate much higher intensity than those produced by the cleavage of alpha2-6 sialyl linkages in sialyllactoses and sialyl-N-acetyllactosamines. Thus, alpha2-3 sialyl linkages cleave much easier than alpha2-6 sialyl linkages in MALDI-PSD fragmentation method. These results suggest that the new techniques using PSD fragmentation of MALDI-TOF mass spectrometry enables us to distinguish alpha2-3 sialyl linkage from alpha2-6 linkage in sialyl oligosaccharides.lld:pubmed
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pubmed-article:10737323pubmed:authorpubmed-author:NakanishiHHlld:pubmed
pubmed-article:10737323pubmed:authorpubmed-author:YamagakiTTlld:pubmed
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pubmed-article:10737323pubmed:volume16lld:pubmed
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pubmed-article:10737323pubmed:pagination385-9lld:pubmed
pubmed-article:10737323pubmed:dateRevised2000-12-18lld:pubmed
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pubmed-article:10737323pubmed:articleTitleA new technique distinguishing alpha2-3 sialyl linkage from alpha2-6 linkage in sialyllactoses and sialyl-N-acetyllactosamines by post-source decay fragmentation method of MALDI-TOF mass spectrometry.lld:pubmed
pubmed-article:10737323pubmed:affiliationNational Institute of Bioscience & Human-Technology, Tsukuba, Ibaraki, Japan.lld:pubmed
pubmed-article:10737323pubmed:publicationTypeJournal Articlelld:pubmed
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