Linked Life Data

10737323

Source:http://linkedlifedata.com/resource/pubmed/id/10737323

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2000-5-19
pubmed:abstractText
Alpha2-3 and alpha2-6 sialyl linkage types of sialyllactoses and sialyl-N-acetyllactosamines were analyzed by post-source decay (PSD) fragmentation method using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. A new matrix of norharmane was suited for the MALDI-TOF measurements of sialyl oligosaccharides. The fragment ions B1 produced by the cleavage of alpha2-3 sialyl linkages indicate much higher intensity than those produced by the cleavage of alpha2-6 sialyl linkages in sialyllactoses and sialyl-N-acetyllactosamines. Thus, alpha2-3 sialyl linkages cleave much easier than alpha2-6 sialyl linkages in MALDI-PSD fragmentation method. These results suggest that the new techniques using PSD fragmentation of MALDI-TOF mass spectrometry enables us to distinguish alpha2-3 sialyl linkage from alpha2-6 linkage in sialyl oligosaccharides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0282-0080
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
385-9
pubmed:dateRevised
2000-12-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
A new technique distinguishing alpha2-3 sialyl linkage from alpha2-6 linkage in sialyllactoses and sialyl-N-acetyllactosamines by post-source decay fragmentation method of MALDI-TOF mass spectrometry.
pubmed:affiliation
National Institute of Bioscience & Human-Technology, Tsukuba, Ibaraki, Japan.
pubmed:publicationType
Journal Article