Linked Life Data

10733933

Source:http://linkedlifedata.com/resource/pubmed/id/10733933

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-5-4
pubmed:abstractText
Caco-2 cells undergo differentiation to an enterocytic-like cell when maintained in a post-confluent state for 1-2 weeks. During this period Caco-2 cells begin to express high levels brush border membrane associated enzymes such as dipeptidyl peptidase IV. Using the dipeptidyl peptidase IV gene promoter in electrophoretic mobility shift assays, we have shown for the first time that levels of hepatocyte nuclear factor 1alpha increase three- to fourfold during Caco-2 cell differentiation. Transient cotransfection experiments with 3T3 cells using dipeptidyl peptidase IV promoter constructs and expression vectors containing hepatocyte nuclear factor 1alpha and beta show that the ratio of alpha and beta modulates reporter gene expression. These results suggest that the increase in levels of hepatocyte nuclear factor 1alpha that occur during intestinal cell differentiation, are important for expression of dipeptidyl peptidase IV and other intestinal proteins.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Academic Press.
pubmed:issnType
Print
pubmed:day
2
pubmed:volume
270
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
235-9
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:10733933-Caco-2 Cells, pubmed-meshheading:10733933-Cell Differentiation, pubmed-meshheading:10733933-Colonic Neoplasms, pubmed-meshheading:10733933-DNA-Binding Proteins, pubmed-meshheading:10733933-Dipeptidyl Peptidase 4, pubmed-meshheading:10733933-Enterocytes, pubmed-meshheading:10733933-Gene Expression Regulation, Enzymologic, pubmed-meshheading:10733933-Gene Expression Regulation, Neoplastic, pubmed-meshheading:10733933-Hepatocyte Nuclear Factor 1, pubmed-meshheading:10733933-Hepatocyte Nuclear Factor 1-alpha, pubmed-meshheading:10733933-Hepatocyte Nuclear Factor 1-beta, pubmed-meshheading:10733933-Humans, pubmed-meshheading:10733933-Intestine, Small, pubmed-meshheading:10733933-Microvilli, pubmed-meshheading:10733933-Nuclear Proteins, pubmed-meshheading:10733933-Transcription, Genetic, pubmed-meshheading:10733933-Transcription Factors
pubmed:year
2000
pubmed:articleTitle
Role of hepatocyte nuclear factor 1alpha and 1beta in the transcriptional regulation of human dipeptidyl peptidase IV during differentiation of Caco-2 cells.
pubmed:affiliation
Gastrointestinal Research Laboratory, Department of Veterans Affairs Medical Center, San Francisco, California 94121, USA. neko@itsa.ucsf.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.