Linked Life Data

10732676

Source:http://linkedlifedata.com/resource/pubmed/id/10732676

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-4-5
pubmed:abstractText
Site-specific recombination within the Saccharomyces cerevisiae 2-micron DNA plasmid is catalyzed by the Flp recombinase at specific Flp Recognition Target (FRT) sites, which lie near the center of two precise 599-bp Inverted Repeats (IRs). However, the role of IR DNA sequences other than the FRT itself for the function of the Flp reaction in vivo is not known. In the present work we report that recombination efficiency differs depending on whether the FRT or the entire IR serves as the substrate for Flp. We also provide evidence for the involvement of the IR in RAD52-dependent homologous recombination. In contrast, the catalysis of site-specific recombination between two FRTs does not require the function of RAD52. The efficiency of Flp site-specific recombination between two IRs cloned in the same orientation is about one hundred times higher than that obtained when only the two FRTs are present. Moreover, we demonstrate that a single IR can activate RAD52-dependent homologous recombination between two flanking DNA regions, providing new insights into the role of the IR as a substrate for recombination and a new experimental tool with which to study the molecular mechanism of homologous recombination.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0026-8925
pubmed:author
pubmed:issnType
Print
pubmed:volume
263
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10732676-Base Sequence, pubmed-meshheading:10732676-Binding Sites, pubmed-meshheading:10732676-DNA, Fungal, pubmed-meshheading:10732676-DNA Nucleotidyltransferases, pubmed-meshheading:10732676-DNA Primers, pubmed-meshheading:10732676-DNA-Binding Proteins, pubmed-meshheading:10732676-Fungal Proteins, pubmed-meshheading:10732676-Genes, Fungal, pubmed-meshheading:10732676-Molecular Sequence Data, pubmed-meshheading:10732676-Plasmids, pubmed-meshheading:10732676-Rad52 DNA Repair and Recombination Protein, pubmed-meshheading:10732676-Recombination, Genetic, pubmed-meshheading:10732676-Repetitive Sequences, Nucleic Acid, pubmed-meshheading:10732676-Saccharomyces cerevisiae, pubmed-meshheading:10732676-Saccharomyces cerevisiae Proteins, pubmed-meshheading:10732676-Substrate Specificity
pubmed:year
2000
pubmed:articleTitle
Involvement of the inverted repeat of the yeast 2-micron plasmid in Flp site-specific and RAD52-dependent homologous recombination.
pubmed:affiliation
Microbiology Group, ICGEB, AREA Science Park, Trieste, Italy.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't