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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
2000-4-26
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pubmed:abstractText |
To establish a sensitive and specific antibody assay, potent antigenic proteins encoded by human herpesvirus 8 (HHV8) were studied. Fifteen recombinant HHV8-encoded proteins were produced as glutathione S-transferase fusion proteins. The sera from AIDS-associated Kaposi's sarcoma (KS) patients reacted with four proteins encoded by open reading frames (ORFs) K8.1, 59, 65, and 73 in a Western blot assay. An enzyme-linked immunosorbent assay (ELISA) using these four proteins as antigens (mixed-antigen ELISA) revealed that all 26 sera derived from KS patients (24 with and 2 without human immunodeficiency virus infection) became positive for anti-HHV8 antibodies. The presence of HHV8 was demonstrated in 14 (1. 4%) of 1,004 sera from the Japanese general population and 10 (1.9%) of 527 sera from patients without HHV8-associated diseases. The presence of immunoglobulin G (IgG) and IgM antibodies against HHV8 examined further by the mixed-antigen ELISA and Western blotting revealed IgG antibody in all ELISA-positive sera, while IgM antibody against ORF K8.1 was absent. These data suggest that the ORF 73 and 65 proteins are potent antigens for a sensitive serological assay.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-10068601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-10191203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-10393835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-10421407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-10502268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-3047011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-7632932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-7700310,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-7997879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-8657239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-8695812,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-8888167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-8962146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9020272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9060668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9083932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9129068,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9223481,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9527930,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9620379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9620422,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9639499,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9658120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9679756,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9697708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9714046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9733875,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9740785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10729121-9882339
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
74
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3478-85
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10729121-AIDS-Related Opportunistic Infections,
pubmed-meshheading:10729121-Adolescent,
pubmed-meshheading:10729121-Adult,
pubmed-meshheading:10729121-Antibodies, Viral,
pubmed-meshheading:10729121-Antigens, Viral,
pubmed-meshheading:10729121-Blotting, Western,
pubmed-meshheading:10729121-Child,
pubmed-meshheading:10729121-Child, Preschool,
pubmed-meshheading:10729121-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10729121-Female,
pubmed-meshheading:10729121-Herpesviridae Infections,
pubmed-meshheading:10729121-Herpesvirus 8, Human,
pubmed-meshheading:10729121-Humans,
pubmed-meshheading:10729121-Infant,
pubmed-meshheading:10729121-Infant, Newborn,
pubmed-meshheading:10729121-Male,
pubmed-meshheading:10729121-Middle Aged,
pubmed-meshheading:10729121-Open Reading Frames,
pubmed-meshheading:10729121-Recombinant Fusion Proteins,
pubmed-meshheading:10729121-Sarcoma, Kaposi,
pubmed-meshheading:10729121-Sensitivity and Specificity,
pubmed-meshheading:10729121-Seroepidemiologic Studies,
pubmed-meshheading:10729121-Viral Proteins
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pubmed:year |
2000
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pubmed:articleTitle |
Identification of antigenic proteins encoded by human herpesvirus 8 and seroprevalence in the general population and among patients with and without Kaposi's sarcoma.
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pubmed:affiliation |
Department of Pathology, AIDS Research Center, National Institute of Infectious Diseases, Shinjuku, Tokyo 162-8640, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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