Linked Life Data

10728975

Source:http://linkedlifedata.com/resource/pubmed/id/10728975

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-4-4
pubmed:abstractText
Human gingival crevicular fluid contains unidentified proteins which might play a role as markers in periodontal diseases. Therefore, low-molecular-weight proteins found in human gingival crevicular fluid (GCF), but absent from serum, were identified in the present study by means of two-dimensional electrophoresis (2-D PAGE) analysis. GCF, serum, and whole saliva were collected from periodontitis and healthy subjects, as well as from edentulous and newborn subjects. Protein samples were separated by two-dimensional polyacrylamide gel electrophoresis, stained with silver, and compared with reference protein maps in the SWISS-2D PAGE database. In GCF and saliva from periodontitis patients and healthy subjects, four dominant low-molecular-mass (from 8 to 14 kDa) acidic spots were observed. They were not found in serum and were less visible in saliva from edentulous and newborn subjects. From N-terminal amino acid sequencing, the two 2-D protein spots of 8 kDa and isoelectric points between 6.5 and 7.0 were both identified as protein MRP8 (SI00A8), a member of the S100 family of calcium-binding proteins. Using peptide mass fingerprinting and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS), we identified the other two protein spots, with mass of 14 kDa and isoelectric points between 5.5 and 6.0, as protein MRP14 (S100A9), also belonging to the S100 family. The presence of MRP8 and MRP14 in GCF was confirmed by Western blot, with monoclonal antibodies. The two polypeptides, MRP8 and MRP14, identified in GCF represent the major difference between the 2-D PAGE patterns of serum and GCF, and we hypothesize that they may play an important role in the gingival sulcus and could represent possible markers for periodontal diseases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-0345
pubmed:author
pubmed:issnType
Print
pubmed:volume
79
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
740-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10728975-Adult, pubmed-meshheading:10728975-Aged, pubmed-meshheading:10728975-Antigens, Differentiation, pubmed-meshheading:10728975-Biological Markers, pubmed-meshheading:10728975-Blotting, Western, pubmed-meshheading:10728975-Calcium-Binding Proteins, pubmed-meshheading:10728975-Calgranulin A, pubmed-meshheading:10728975-Calgranulin B, pubmed-meshheading:10728975-Coloring Agents, pubmed-meshheading:10728975-Electrophoresis, Gel, Two-Dimensional, pubmed-meshheading:10728975-Gingival Crevicular Fluid, pubmed-meshheading:10728975-Humans, pubmed-meshheading:10728975-Infant, pubmed-meshheading:10728975-Infant, Newborn, pubmed-meshheading:10728975-Isoelectric Point, pubmed-meshheading:10728975-Lasers, pubmed-meshheading:10728975-Mass Spectrometry, pubmed-meshheading:10728975-Middle Aged, pubmed-meshheading:10728975-Molecular Weight, pubmed-meshheading:10728975-Mouth, pubmed-meshheading:10728975-Mouth, Edentulous, pubmed-meshheading:10728975-Peptide Mapping, pubmed-meshheading:10728975-Periodontitis, pubmed-meshheading:10728975-S100 Proteins, pubmed-meshheading:10728975-Saliva, pubmed-meshheading:10728975-Sequence Analysis, Protein, pubmed-meshheading:10728975-Silver
pubmed:year
2000
pubmed:articleTitle
Human gingival crevicular fluid contains MRP8 (S100A8) and MRP14 (S100A9), two calcium-binding proteins of the S100 family.
pubmed:affiliation
Division of Oral Physiopathology and Periodontology, School of Dentistry, Faculty of Medicine, University of Geneva, Switzerland.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't