| pubmed-article:10727708 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:10727708 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:10727708 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:10727708 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:10727708 | lifeskim:mentions | umls-concept:C0521119 | lld:lifeskim |
| pubmed-article:10727708 | lifeskim:mentions | umls-concept:C0206518 | lld:lifeskim |
| pubmed-article:10727708 | lifeskim:mentions | umls-concept:C0427965 | lld:lifeskim |
| pubmed-article:10727708 | lifeskim:mentions | umls-concept:C1412517 | lld:lifeskim |
| pubmed-article:10727708 | lifeskim:mentions | umls-concept:C1423613 | lld:lifeskim |
| pubmed-article:10727708 | lifeskim:mentions | umls-concept:C1720675 | lld:lifeskim |
| pubmed-article:10727708 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:10727708 | pubmed:dateCreated | 2000-5-25 | lld:pubmed |
| pubmed-article:10727708 | pubmed:abstractText | We have examined the sensitivity of human and rat homo-oligomeric rho(1) GABA receptors to variations in extracellular pH (pH(o)) using the whole-cell patch clamp technique. The GABA-induced conductance mediated by the rat rho(1) receptor (rho(1)-R) decreased with a decrease in pH(o) between 9.0 to 5.4. Below pH(o) 7.4 the effect of protons on the GABA-induced conductance was apparently competitive, but above pH(o) 7.4 the inhibitory effect of extracellular protons was almost independent on the GABA concentration. Titration of the GABA-induced conductance at 3 microM GABA revealed two protonation sites on rat rho(1)-R with pKa 6.4 and pKa 8.2. At 10 microM GABA the low pKa (6.4) was shifted to a clearly lower value (5.6), but the high pKa was only slightly decreased (from 8.2 to 7.9). Zn(2+) ions were capable of relieving the proton inhibition at low pH(o) indicating that Zn(2+) interacts with the low pKa site. Unlike the rat rho(1)-R, the human rho(1)-R was sensitive only to changes in pH(o) at acidic levels. Proton inhibition of human rho(1)-R was apparently competitive, as observed on rat-rho(1) at acidic pH(o). Titration of the human rho(1)-R gave a single H(+) binding site with a pKa of 6.3, similar to the value for the low pKa on rat rho(1)-R. The pKa value of human rho(1)-R was not dependent on the GABA concentration. A chimeric receptor, consisting of the N-terminal part of the rat rho(1)-R and C-terminal part of the human rho(1)-R, displayed pH(o) sensitivity similar to that observed for rat rho(1)-R. This indicates that the high pKa of rat rho(1)-R is attributable to the 11 amino acid differences between the rat and human rho(1)-R extracellular domains. | lld:pubmed |
| pubmed-article:10727708 | pubmed:language | eng | lld:pubmed |
| pubmed-article:10727708 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10727708 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:10727708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10727708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10727708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10727708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10727708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10727708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10727708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10727708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10727708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10727708 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:10727708 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:10727708 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:10727708 | pubmed:issn | 0028-3908 | lld:pubmed |
| pubmed-article:10727708 | pubmed:author | pubmed-author:KailaKK | lld:pubmed |
| pubmed-article:10727708 | pubmed:author | pubmed-author:WegeliusKK | lld:pubmed |
| pubmed-article:10727708 | pubmed:author | pubmed-author:RiveraCC | lld:pubmed |
| pubmed-article:10727708 | pubmed:author | pubmed-author:ReebenMM | lld:pubmed |
| pubmed-article:10727708 | pubmed:author | pubmed-author:Michael... | lld:pubmed |
| pubmed-article:10727708 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:10727708 | pubmed:day | 3 | lld:pubmed |
| pubmed-article:10727708 | pubmed:volume | 39 | lld:pubmed |
| pubmed-article:10727708 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:10727708 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:10727708 | pubmed:pagination | 977-89 | lld:pubmed |
| pubmed-article:10727708 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:10727708 | pubmed:year | 2000 | lld:pubmed |
| pubmed-article:10727708 | pubmed:articleTitle | Different sensitivities of human and rat rho(1) GABA receptors to extracellular pH. | lld:pubmed |
| pubmed-article:10727708 | pubmed:affiliation | Department of Biosciences, Division of Animal Physiology, P.O. Box 17, FIN-00014 University of Helsinki, Finland. | lld:pubmed |
| pubmed-article:10727708 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:10727708 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:10727708 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:10727708 | lld:pubmed |
