Linked Life Data

10727630

Source:http://linkedlifedata.com/resource/pubmed/id/10727630

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2000-6-15
pubmed:abstractText
Neurons from many brainstem nuclei involved in respiratory control increase their firing rate in response to acidosis in vitro, suggesting that they are central chemoreceptors. This property has been considered to be either unique to neurons involved in respiratory control, or at least very unusual for non-respiratory neurons. However, recordings of intrinsic pH responses of neurons have not been made from enough non-respiratory regions of the CNS to be certain this assumption is true. Here, we have quantified changes in firing rate of neurons cultured from the hippocampus (n=43), neocortex (n=33), and cerebellum (n=29) in response to changes in CO(2) between 3% and 9% (pH approximately 7.6-7.2) after blockade of glutamatergic and GABAergic transmission. The responses of neurons from these three regions were similar, with a subset of neurons (12% of the total 105) inhibited by acidosis, decreasing their firing rate to a mean of 70% of control in response to a decrease in pH of 0.2. Some neurons (5% of total) were stimulated by acidosis, with an increase in firing rate to a mean of 175% of control in response to a decrease in pH of 0.2. We previously quantified chemosensitivity of neurons from the medullary raphe using the same methods [W. Wang, J.H. Pizzonia, G.B. Richerson, Chemosensitivity of rat medullary raphe neurones in primary tissue culture, J. Physiol., 511 (1998) 433-450]. Compared to these non-respiratory neurons, more raphe neurons were stimulated by acidosis (22%), and the average response was greater (to 300% of control) in response to the same stimulus. Thus, over a physiologically relevant pH range, stimulation by acidosis occurs in a significant percentage of neurons not involved in respiratory chemoreception. However, the degree of chemosensitivity of these neurons was less than medullary raphe neurons under the same conditions. Chemosensitivity is not an all-or-none neuronal property, and the degree of chemosensitivity may be relevant to the role neurons play in sensing pH in vivo.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0006-8993
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
860
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
119-29
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10727630-2-Amino-5-phosphonovalerate, pubmed-meshheading:10727630-6-Cyano-7-nitroquinoxaline-2,3-dione, pubmed-meshheading:10727630-Acidosis, pubmed-meshheading:10727630-Action Potentials, pubmed-meshheading:10727630-Animals, pubmed-meshheading:10727630-Animals, Newborn, pubmed-meshheading:10727630-Cells, Cultured, pubmed-meshheading:10727630-Cerebellum, pubmed-meshheading:10727630-Chemoreceptor Cells, pubmed-meshheading:10727630-Fibroblast Growth Factor 2, pubmed-meshheading:10727630-Fibroblast Growth Factor 5, pubmed-meshheading:10727630-Fibroblast Growth Factors, pubmed-meshheading:10727630-Hippocampus, pubmed-meshheading:10727630-Hydrogen-Ion Concentration, pubmed-meshheading:10727630-Neocortex, pubmed-meshheading:10727630-Neurons, pubmed-meshheading:10727630-Raphe Nuclei, pubmed-meshheading:10727630-Rats, pubmed-meshheading:10727630-Respiratory Center
pubmed:year
2000
pubmed:articleTitle
Chemosensitivity of non-respiratory rat CNS neurons in tissue culture.
pubmed:affiliation
Department of Neurology, West Haven, CT, USA. george.richerson@yale.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't