Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2000-3-31
pubmed:abstractText
NF-kappa B plays a pivotal role in normal T-cell activation and may also mediate human T-cell leukemia virus (HTLV)-induced T-cell transformation. Activation of NF-kappa B by both T-cell costimulatory signals and the HTLV Tax protein involves stimulation of I kappa B kinase (IKK). As a genetic approach to dissect the intermediate steps involved in NF-kappa B activation in human T cells, we performed somatic cell mutagenesis to isolate signaling-defective mutant Jurkat T-cell lines. One of the mutant cell lines was shown to have a specific blockade in the IKK signaling pathway but remained competent in the c-Jun N-terminal kinase and MAP kinase pathways. Interestingly, this mutant cell line lacks expression of IKK gamma, a non-catalytic component of the IKK complex. Expression of exogenous IKK gamma in the mutant cells restored NF-kappa B activation by both the T-cell costimulation agents and Tax. These findings provide genetic evidence for the requirement of IKK gamma in NF-kappa B signaling triggered by both T-cell costimulatory signals and HTLV-I Tax protein.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3, http://linkedlifedata.com/resource/pubmed/chemical/CHUK protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tax, http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Kinase, http://linkedlifedata.com/resource/pubmed/chemical/IKBKB protein, human, http://linkedlifedata.com/resource/pubmed/chemical/IKBKE protein, human, http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein..., http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Mitogens, http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0950-9232
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1448-56
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10723136-Antibodies, Monoclonal, pubmed-meshheading:10723136-Antigens, CD28, pubmed-meshheading:10723136-Antigens, CD3, pubmed-meshheading:10723136-Cell Line, pubmed-meshheading:10723136-Cell Separation, pubmed-meshheading:10723136-Gene Products, tax, pubmed-meshheading:10723136-Human T-lymphotropic virus 1, pubmed-meshheading:10723136-Humans, pubmed-meshheading:10723136-I-kappa B Kinase, pubmed-meshheading:10723136-JNK Mitogen-Activated Protein Kinases, pubmed-meshheading:10723136-Jurkat Cells, pubmed-meshheading:10723136-Lymphocyte Activation, pubmed-meshheading:10723136-Mitogen-Activated Protein Kinases, pubmed-meshheading:10723136-Mitogens, pubmed-meshheading:10723136-Mutagenesis, pubmed-meshheading:10723136-NF-kappa B, pubmed-meshheading:10723136-Phosphorylation, pubmed-meshheading:10723136-Protein-Serine-Threonine Kinases, pubmed-meshheading:10723136-Signal Transduction, pubmed-meshheading:10723136-T-Lymphocytes, pubmed-meshheading:10723136-Tetradecanoylphorbol Acetate
pubmed:year
2000
pubmed:articleTitle
Somatic mutagenesis studies of NF-kappa B signaling in human T cells: evidence for an essential role of IKK gamma in NF-kappa B activation by T-cell costimulatory signals and HTLV-I Tax protein.
pubmed:affiliation
Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, Pennsylvania, PA 17033, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.