rdf:type |
|
lifeskim:mentions |
umls-concept:C0035820,
umls-concept:C0037083,
umls-concept:C0039194,
umls-concept:C0079576,
umls-concept:C0079866,
umls-concept:C0079904,
umls-concept:C0086418,
umls-concept:C0205224,
umls-concept:C0332120,
umls-concept:C1533711,
umls-concept:C1710082,
umls-concept:C1879547,
umls-concept:C2603343
|
pubmed:issue |
11
|
pubmed:dateCreated |
2000-3-31
|
pubmed:abstractText |
NF-kappa B plays a pivotal role in normal T-cell activation and may also mediate human T-cell leukemia virus (HTLV)-induced T-cell transformation. Activation of NF-kappa B by both T-cell costimulatory signals and the HTLV Tax protein involves stimulation of I kappa B kinase (IKK). As a genetic approach to dissect the intermediate steps involved in NF-kappa B activation in human T cells, we performed somatic cell mutagenesis to isolate signaling-defective mutant Jurkat T-cell lines. One of the mutant cell lines was shown to have a specific blockade in the IKK signaling pathway but remained competent in the c-Jun N-terminal kinase and MAP kinase pathways. Interestingly, this mutant cell line lacks expression of IKK gamma, a non-catalytic component of the IKK complex. Expression of exogenous IKK gamma in the mutant cells restored NF-kappa B activation by both the T-cell costimulation agents and Tax. These findings provide genetic evidence for the requirement of IKK gamma in NF-kappa B signaling triggered by both T-cell costimulatory signals and HTLV-I Tax protein.
|
pubmed:grant |
|
pubmed:language |
eng
|
pubmed:journal |
|
pubmed:citationSubset |
IM
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/CHUK protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, tax,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/IKBKB protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/IKBKE protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/JNK Mitogen-Activated Protein...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogens,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate
|
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0950-9232
|
pubmed:author |
|
pubmed:issnType |
Print
|
pubmed:day |
9
|
pubmed:volume |
19
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
1448-56
|
pubmed:dateRevised |
2009-11-19
|
pubmed:meshHeading |
pubmed-meshheading:10723136-Antibodies, Monoclonal,
pubmed-meshheading:10723136-Antigens, CD28,
pubmed-meshheading:10723136-Antigens, CD3,
pubmed-meshheading:10723136-Cell Line,
pubmed-meshheading:10723136-Cell Separation,
pubmed-meshheading:10723136-Gene Products, tax,
pubmed-meshheading:10723136-Human T-lymphotropic virus 1,
pubmed-meshheading:10723136-Humans,
pubmed-meshheading:10723136-I-kappa B Kinase,
pubmed-meshheading:10723136-JNK Mitogen-Activated Protein Kinases,
pubmed-meshheading:10723136-Jurkat Cells,
pubmed-meshheading:10723136-Lymphocyte Activation,
pubmed-meshheading:10723136-Mitogen-Activated Protein Kinases,
pubmed-meshheading:10723136-Mitogens,
pubmed-meshheading:10723136-Mutagenesis,
pubmed-meshheading:10723136-NF-kappa B,
pubmed-meshheading:10723136-Phosphorylation,
pubmed-meshheading:10723136-Protein-Serine-Threonine Kinases,
pubmed-meshheading:10723136-Signal Transduction,
pubmed-meshheading:10723136-T-Lymphocytes,
pubmed-meshheading:10723136-Tetradecanoylphorbol Acetate
|
pubmed:year |
2000
|
pubmed:articleTitle |
Somatic mutagenesis studies of NF-kappa B signaling in human T cells: evidence for an essential role of IKK gamma in NF-kappa B activation by T-cell costimulatory signals and HTLV-I Tax protein.
|
pubmed:affiliation |
Department of Microbiology and Immunology, Pennsylvania State University College of Medicine, 500 University Drive, Hershey, Pennsylvania, PA 17033, USA.
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|