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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-5-3
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pubmed:abstractText |
In this study we have examined the effect of the SNARE protein syntaxin 1A on the glycine transporters GLYT1 and GLYT2. Our results demonstrate a functional and physical interaction between both glycine transporters and syntaxin 1A. Co-transfection of syntaxin 1A with GLYT1 or GLYT2 in COS cells resulted in approximately 40% inhibition in glycine transport. This inhibition was reversed by the syntaxin 1A-binding protein, Munc18. Furthermore, immunoprecipitation studies showed a physical interaction between syntaxin 1A and both transporters in COS cells and in rat brain tissue. Finally, we conclude that this physical interaction resulted in a partial removal of the glycine transporters from the plasma membrane as demonstrated by biotinylation studies.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acid Transport Systems...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycine Plasma Membrane Transport...,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Slc6a5 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Slc6a9 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Snap25 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Stx1a protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Synaptosomal-Associated Protein 25,
http://linkedlifedata.com/resource/pubmed/chemical/Syntaxin 1
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
470
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
51-4
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10722844-Amino Acid Transport Systems, Neutral,
pubmed-meshheading:10722844-Animals,
pubmed-meshheading:10722844-Antigens, Surface,
pubmed-meshheading:10722844-COS Cells,
pubmed-meshheading:10722844-Carrier Proteins,
pubmed-meshheading:10722844-Cell Membrane,
pubmed-meshheading:10722844-Glycine Plasma Membrane Transport Proteins,
pubmed-meshheading:10722844-Membrane Proteins,
pubmed-meshheading:10722844-Nerve Tissue Proteins,
pubmed-meshheading:10722844-Rats,
pubmed-meshheading:10722844-Synaptosomal-Associated Protein 25,
pubmed-meshheading:10722844-Syntaxin 1
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pubmed:year |
2000
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pubmed:articleTitle |
Characterization of the interactions between the glycine transporters GLYT1 and GLYT2 and the SNARE protein syntaxin 1A.
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pubmed:affiliation |
Centro de Biología Molecular 'Severo Ochoa', Facultad de Ciencias, Universidad Autónoma de Madrid, Consejo Superior de Investigaciones Científicas, 28049, Madrid, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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