Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-5-3
pubmed:abstractText
Mesenchyme forkhead-1 (MFH-1), a winged helix/forkhead transcription factor, is expressed in developing cartilaginous tissues, kidney and arch arteries, and is essential for the normal development of the axial skeleton and aortic arch formation of mice. To investigate the possible role of MFH-1 in osteogenesis and osteoblast differentiation, we examined expression of MFH-1 induced by bone morphogenetic protein-2 (BMP-2) in C2C12 myoblasts, and found that MFH-1 protein and also MFH-1 mRNA increased markedly in C2C12 cells after treatment with BMP-2. To confirm the hypothesis that BMP-2 induced osteoblastic differentiation of C2C12 cells by increasing MFH-1 expression, we lowered the endogenous MFH-1 level by stably transfecting C2C12 cells with antisense MFH-1 sequence, and found that in antisense MFH-1 cell lines, both alkaline phosphatase (ALP) activity and production of osteocalcin induced by BMP-2 decreased markedly in comparison with control cell lines. Our results suggest that the BMP-2-induced MFH-1 protein may play a key role in regulating the commitment to osteoblastic differentiation of C2C12 myoblasts and production of osteoblast markers including ALP and osteocalcin.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, http://linkedlifedata.com/resource/pubmed/chemical/Bmp2 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Bmp2 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 2, http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Forkhead Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta, http://linkedlifedata.com/resource/pubmed/chemical/mesenchyme fork head 1 protein
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
470
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
29-34
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10722840-Animals, pubmed-meshheading:10722840-Antibodies, Monoclonal, pubmed-meshheading:10722840-Antibody Specificity, pubmed-meshheading:10722840-Bone Morphogenetic Protein 2, pubmed-meshheading:10722840-Bone Morphogenetic Proteins, pubmed-meshheading:10722840-Cell Differentiation, pubmed-meshheading:10722840-Cell Line, pubmed-meshheading:10722840-DNA-Binding Proteins, pubmed-meshheading:10722840-Forkhead Transcription Factors, pubmed-meshheading:10722840-Gene Expression, pubmed-meshheading:10722840-L Cells (Cell Line), pubmed-meshheading:10722840-Mice, pubmed-meshheading:10722840-Oligonucleotides, Antisense, pubmed-meshheading:10722840-Osteoblasts, pubmed-meshheading:10722840-RNA, Messenger, pubmed-meshheading:10722840-Rats, pubmed-meshheading:10722840-Rats, Wistar, pubmed-meshheading:10722840-Transcription Factors, pubmed-meshheading:10722840-Transfection, pubmed-meshheading:10722840-Transforming Growth Factor beta
pubmed:year
2000
pubmed:articleTitle
MFH-1 is required for bone morphogenetic protein-2-induced osteoblastic differentiation of C2C12 myoblasts.
pubmed:affiliation
Department of Biochemistry, Akita University School of Medicine, Hondo 1-1-1, Akita, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't