Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-4-20
pubmed:abstractText
Histoplasma capsulatum induces a cell-mediated immune response in lungs and lymphoid organs of mammals. Resolution of primary infection in mice depends on interleukin-12 (IL-12), since neutralization of this monokine increases susceptibility to infection. The present study was designed to determine if blockade of IL-12 disrupts the protective immune response by altering the influx of lineage-specific cells into infected lungs and the numbers of cells expressing CD80, CD86, CD119, and major histocompatibility complex class II (MHC II) molecules. In mice given anti-IL-12, there was a 2.5-fold decrease in total numbers of T cells on days 3 to 10 of infection and a 4-fold increase in Mac-1/Gr-1(+) cells on days 7 and 10 compared to infected controls. CD80(+) lung cells from anti-IL-12-treated mice were 2- to 3-fold greater than those from controls on days 7 and 10, whereas the total numbers of CD86(+) cells were 2- to 3-fold less and MHC II(+) cells were 1.5- to 2-fold less on days 3 and 5. Cells expressing CD119 were reduced 1.5-fold on day 5. Treatment with monoclonal antibodies (MAb) to CD80, CD86, or both reduced the fungal burden slightly compared to that in rat immunoglobulin G-treated controls, whereas after IL-12 neutralization, blocking of CD80 reduced the tissue burden by 2. 5-fold and this correlated with a decrease in IL-4. Regardless, mortality was not altered by treatment with MAb to CD80 or CD86. We conclude that (i) IL-12 neutralization alters the nature of the inflammatory response in lungs and the expression of CD80 and CD86 on lineage-specific cells, (ii) the immune response during infection with H. capsulatum is controlled via mechanisms independent of the CD80 and CD86 costimulatory pathways, and (iii) decreased expression of CD86 and MHC II may modulate generation of optimal protective immunity.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-10229856, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-10359580, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-10403924, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-1590984, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-2509369, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-3025331, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-3805742, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-7523517, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-7525726, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-7534215, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-7538442, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-7608555, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-7614983, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-7687627, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-7876554, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-796385, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-8301195, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-8609397, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-8799723, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-8940222, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-8943375, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-8945587, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-9039773, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-9086148, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-9151904, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-9199420, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-9379045, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-9423867, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-9529070, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-9677302, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-9720957, http://linkedlifedata.com/resource/pubmed/commentcorrection/10722603-9834110
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86, http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage..., http://linkedlifedata.com/resource/pubmed/chemical/Histocompatibility Antigens Class II, http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G, http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0019-9567
pubmed:author
pubmed:issnType
Print
pubmed:volume
68
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2069-76
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:10722603-Animals, pubmed-meshheading:10722603-Mice, pubmed-meshheading:10722603-Leukocytes, pubmed-meshheading:10722603-Pneumonia, pubmed-meshheading:10722603-Rats, pubmed-meshheading:10722603-Histoplasmosis, pubmed-meshheading:10722603-Male, pubmed-meshheading:10722603-Time Factors, pubmed-meshheading:10722603-Fluorescent Antibody Technique, pubmed-meshheading:10722603-Immunoglobulin G, pubmed-meshheading:10722603-Mice, Inbred C57BL, pubmed-meshheading:10722603-Histocompatibility Antigens Class II, pubmed-meshheading:10722603-Antibodies, Monoclonal, pubmed-meshheading:10722603-Antigens, CD, pubmed-meshheading:10722603-Membrane Glycoproteins, pubmed-meshheading:10722603-Interferon-gamma, pubmed-meshheading:10722603-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:10722603-Immunophenotyping, pubmed-meshheading:10722603-Tumor Necrosis Factor-alpha, pubmed-meshheading:10722603-Hybridomas, pubmed-meshheading:10722603-Interleukin-4, pubmed-meshheading:10722603-Cell Lineage
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