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rdf:type |
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lifeskim:mentions |
umls-concept:C0010453,
umls-concept:C0018956,
umls-concept:C0021757,
umls-concept:C0085732,
umls-concept:C0125606,
umls-concept:C0128546,
umls-concept:C0143630,
umls-concept:C0591833,
umls-concept:C1366480,
umls-concept:C1522012,
umls-concept:C1948023,
umls-concept:C1998793,
umls-concept:C2003941,
umls-concept:C2350701
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pubmed:issue |
4
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pubmed:dateCreated |
2000-4-5
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pubmed:abstractText |
The engraftment potential of murine stem cells (HSC) is greatly reduced when these cells are expanded in vitro with stem cell factor and interleukin-3. We have evaluated if the addition of MIP-1 alpha or LIF to these cultures would protect the ability of murine wild type HSC to engraft the stem cell defective W/Wv recipient. In this transplantation model red and white blood cell reconstitution is assessed by hemoglobin electrophoresis and c-kit PCR genotyping, respectively. The results obtained indicate that both MIP-1 alpha and LIF protect, at least transiently, the HSC repopulating ability in vivo in spite of the modest expansion in the number of nucleated and progenitor cells observed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Chemokine CCL4,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-3,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Leukemia Inhibitory Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Lif protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Lymphokines,
http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Inflammatory Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor
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pubmed:status |
MEDLINE
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pubmed:issn |
0021-2571
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
35
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
553-62
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10721226-Analysis of Variance,
pubmed-meshheading:10721226-Animals,
pubmed-meshheading:10721226-Cell Division,
pubmed-meshheading:10721226-Cell Separation,
pubmed-meshheading:10721226-Chemokine CCL4,
pubmed-meshheading:10721226-Culture Media, Serum-Free,
pubmed-meshheading:10721226-Female,
pubmed-meshheading:10721226-Genotype,
pubmed-meshheading:10721226-Growth Inhibitors,
pubmed-meshheading:10721226-Hematopoietic Stem Cells,
pubmed-meshheading:10721226-Interleukin-3,
pubmed-meshheading:10721226-Interleukin-6,
pubmed-meshheading:10721226-Leukemia Inhibitory Factor,
pubmed-meshheading:10721226-Lymphokines,
pubmed-meshheading:10721226-Macrophage Inflammatory Proteins,
pubmed-meshheading:10721226-Mice,
pubmed-meshheading:10721226-Mice, Inbred C57BL,
pubmed-meshheading:10721226-Polymerase Chain Reaction,
pubmed-meshheading:10721226-Recombinant Proteins,
pubmed-meshheading:10721226-Stem Cell Factor,
pubmed-meshheading:10721226-Stimulation, Chemical,
pubmed-meshheading:10721226-Time Factors
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pubmed:year |
1999
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pubmed:articleTitle |
Macrophage inflammatory protein-1 alpha (MIP-1 alpha) and leukemia inhibitory factor (LIF) protect the repopulating ability of purified murine hematopoietic stem cells in serum-deprived cultures stimulated with SCF and IL-3.
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pubmed:affiliation |
Laboratory of Hematopoietic Growth Factors, Lindsley F. Kimball Research Institute, New York Blood Center, New York, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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