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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2000-3-31
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pubmed:abstractText |
WSU-CLL cells, a fludarabine resistant B-cell chronic lymphocytic leukemia cell line, has been shown to exhibit enhanced sensitivity to 2-chlorodeoxyadenosine (2-CdA) following 48-72 h exposure to bryostatin 1. For 2-CdA to manifest its chemotherapeutic activity, it must first enter the cell through one of several specific nucleoside transporter systems. We present data to show that bryostatin 1-induced enhanced influx of 2-CdA is in part the result of bryostatin 1-induced modulation of nucleoside transporters in WSU-CLL cells. The bi-directional equilibrative NBMPR sensitive transporters in WSU-CLL cells were significantly down-regulated 90 min post-exposure to 1-200 nM bryostatin 1. This down-regulation was evident up to 144 h. In contrast, WSU-CLL cells exhibited a transient increase in Na+-dependent concentrative 2-CdA influx from 48 to 96 h after bryostatin 1 exposure which was evident for a longer duration than that accounted for by the increase in deocycytidine kinase activity. These data may, in part, explain the enhanced efficacy of 2-CdA seen in WSU-CLL cells following 48-72 h exposure to bryostatin 1. It may raise questions as to the importance of the bi-directional transporters in determining the resistance or sensitivity of CLL cells to 2-CdA or other nucleoside analogues.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-nitrobenzylthioinosine,
http://linkedlifedata.com/resource/pubmed/chemical/Antineoplastic Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Bryostatins,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cladribine,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxycytidine Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/Dipyridamole,
http://linkedlifedata.com/resource/pubmed/chemical/Lactones,
http://linkedlifedata.com/resource/pubmed/chemical/Macrolides,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nucleoside Transport Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Thioinosine,
http://linkedlifedata.com/resource/pubmed/chemical/Vidarabine,
http://linkedlifedata.com/resource/pubmed/chemical/bryostatin 1,
http://linkedlifedata.com/resource/pubmed/chemical/fludarabine
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1107-3756
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
341-7
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10719048-Aged,
pubmed-meshheading:10719048-Antineoplastic Agents,
pubmed-meshheading:10719048-Biological Transport, Active,
pubmed-meshheading:10719048-Bryostatins,
pubmed-meshheading:10719048-Carrier Proteins,
pubmed-meshheading:10719048-Cladribine,
pubmed-meshheading:10719048-Deoxycytidine Kinase,
pubmed-meshheading:10719048-Dipyridamole,
pubmed-meshheading:10719048-Down-Regulation,
pubmed-meshheading:10719048-Drug Resistance, Neoplasm,
pubmed-meshheading:10719048-Humans,
pubmed-meshheading:10719048-Lactones,
pubmed-meshheading:10719048-Leukemia, Lymphocytic, Chronic, B-Cell,
pubmed-meshheading:10719048-Macrolides,
pubmed-meshheading:10719048-Male,
pubmed-meshheading:10719048-Membrane Proteins,
pubmed-meshheading:10719048-Nucleoside Transport Proteins,
pubmed-meshheading:10719048-Phosphorylation,
pubmed-meshheading:10719048-Thioinosine,
pubmed-meshheading:10719048-Tumor Cells, Cultured,
pubmed-meshheading:10719048-Vidarabine
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pubmed:year |
2000
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pubmed:articleTitle |
Bryostatin 1-induced modulation of nucleoside transporters and 2-chlorodeoxyadenosine influx in WSU-CLL cells.
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pubmed:affiliation |
Wayne State University School of Medicine, Detroit, MI 48201, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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