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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-4-18
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pubmed:abstractText |
Neurturin (NTN) is a recently identified homologue of glial-cell-line-derived neurotrophic factor. Both factors promote the survival of dopaminergic (DA) neurons. We investigated the biological activity of mammalian-cell-produced NTN versus purified Escherichia coli-produced NTN. Baby hamster kidney cells were engineered to stably secrete mature human NTN. Mammalian-cell-derived NTN enhanced the activity of embryonic DA neurons in vitro, with greater potency (maximum effect achieved in the picogram range) than purified E. coli-produced NTN. Cell-based delivery of NTN (less than 10 ng/day) was also shown to be biologically active in vivo. These results suggest that mammalian-cell-derived NTN, synthesized de novo and delivered in small quantities to the parenchyma at the target site, may be as active as much larger quantities of purified, E. coli-produced NTN, delivered by other means.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Apomorphine,
http://linkedlifedata.com/resource/pubmed/chemical/Capsules,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Conditioned,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/NRTN protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Growth Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Neurturin,
http://linkedlifedata.com/resource/pubmed/chemical/Nrtn protein, rat
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0014-4886
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
189-93
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10716899-Animals,
pubmed-meshheading:10716899-Apomorphine,
pubmed-meshheading:10716899-Behavior, Animal,
pubmed-meshheading:10716899-Biological Assay,
pubmed-meshheading:10716899-Capsules,
pubmed-meshheading:10716899-Cell Culture Techniques,
pubmed-meshheading:10716899-Cell Survival,
pubmed-meshheading:10716899-Cell Transplantation,
pubmed-meshheading:10716899-Cells, Cultured,
pubmed-meshheading:10716899-Cerebral Ventricles,
pubmed-meshheading:10716899-Corpus Striatum,
pubmed-meshheading:10716899-Cricetinae,
pubmed-meshheading:10716899-Culture Media, Conditioned,
pubmed-meshheading:10716899-Dopamine Agonists,
pubmed-meshheading:10716899-Escherichia coli,
pubmed-meshheading:10716899-Gene Expression,
pubmed-meshheading:10716899-Humans,
pubmed-meshheading:10716899-Kidney,
pubmed-meshheading:10716899-Male,
pubmed-meshheading:10716899-Mammals,
pubmed-meshheading:10716899-Nerve Growth Factors,
pubmed-meshheading:10716899-Neurturin,
pubmed-meshheading:10716899-Rats,
pubmed-meshheading:10716899-Rats, Sprague-Dawley,
pubmed-meshheading:10716899-Rotation,
pubmed-meshheading:10716899-Transfection
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pubmed:year |
2000
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pubmed:articleTitle |
Mammalian-cell-produced neurturin (NTN) is more potent than purified Escherichia coli-produced NTN.
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pubmed:affiliation |
CytoTherapeutics, Inc., 701 George Washington Highway Lincoln, Rhode Island, 02865, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study
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