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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-3
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pubmed:dateCreated |
2000-5-1
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pubmed:abstractText |
We assessed the antioxidative effects of quercetin-feeding on ddY strain male mice by measuring luminol-amplified chemiluminescence that was enhanced by a hydrophilic free radical initiator 2, 2'-azobis(2-amidinopropane) dihydrochloride. Quercetin suppressed chemiluminescent intensity in a dose-dependent manner in vitro with a half-inhibition concentration (IC(50)) of 3x10(-8) M, which was lower than the value of synthetic antioxidant 2, 6-di-tert-butyl-p-cresol (IC(50): 7x10(-7) M). Lysosomal (12000xg pellet) and supernatant fractions obtained from the livers of mice fed a diet containing 0.2% quercetin for 7 days showed more inhibition of chemiluminescent intensity than those from non-treated mice. Quercetin feeding also resulted in the inhibition of lipid peroxidation as determined by a thiobarbituric acid reactive substance test which detected suppression of the release of lysosomal enzymes induced by lipophilic free radical initiator 2, 2'-azobis(2,4-dimethylvaleronitrile). These results probably reflect radical quenching activity of quercetin, indicating that the measurement of chemiluminescence is a useful tool for the assessment of total antioxidant activity in biological systems.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,2'-azobis(2,4-dimethylvaleronitril...,
http://linkedlifedata.com/resource/pubmed/chemical/2,2'-azobis(2-amidinopropane),
http://linkedlifedata.com/resource/pubmed/chemical/Acetylglucosaminidase,
http://linkedlifedata.com/resource/pubmed/chemical/Acid Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Amidines,
http://linkedlifedata.com/resource/pubmed/chemical/Azo Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Butylated Hydroxytoluene,
http://linkedlifedata.com/resource/pubmed/chemical/Luminol,
http://linkedlifedata.com/resource/pubmed/chemical/Nitriles,
http://linkedlifedata.com/resource/pubmed/chemical/Oxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Phenols,
http://linkedlifedata.com/resource/pubmed/chemical/Quercetin,
http://linkedlifedata.com/resource/pubmed/chemical/Thiobarbituric Acid Reactive...
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0378-4274
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
114
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
189-96
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10713484-Acetylglucosaminidase,
pubmed-meshheading:10713484-Acid Phosphatase,
pubmed-meshheading:10713484-Administration, Oral,
pubmed-meshheading:10713484-Amidines,
pubmed-meshheading:10713484-Animals,
pubmed-meshheading:10713484-Azo Compounds,
pubmed-meshheading:10713484-Butylated Hydroxytoluene,
pubmed-meshheading:10713484-Dose-Response Relationship, Drug,
pubmed-meshheading:10713484-Lipid Peroxidation,
pubmed-meshheading:10713484-Liver,
pubmed-meshheading:10713484-Luminescent Measurements,
pubmed-meshheading:10713484-Luminol,
pubmed-meshheading:10713484-Lysosomes,
pubmed-meshheading:10713484-Male,
pubmed-meshheading:10713484-Mice,
pubmed-meshheading:10713484-Mice, Inbred Strains,
pubmed-meshheading:10713484-Nitriles,
pubmed-meshheading:10713484-Oxidants,
pubmed-meshheading:10713484-Phenols,
pubmed-meshheading:10713484-Quercetin,
pubmed-meshheading:10713484-Subcellular Fractions,
pubmed-meshheading:10713484-Thiobarbituric Acid Reactive Substances
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pubmed:year |
2000
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pubmed:articleTitle |
Attenuation of luminol-amplified chemiluminescent intensity and lipid peroxidation in the livers of quercetin-fed mice.
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pubmed:affiliation |
Department of Food Science, Kyoto Women's University, Higashiyama-ku, Kyoto, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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