Source:http://linkedlifedata.com/resource/pubmed/id/10713343
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
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| pubmed:dateCreated |
2000-4-12
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| pubmed:abstractText |
Continuous culture of concanavalin A (Con A)-activated spleen cells in the presence of chicken recombinant IL-2 (rIL-2) promoted preferential growth of gammadelta T-cells. These cells displayed a high level of spontaneous cytotoxicity against LSCC-RP9 tumor cells, an avian NK cell target. Stimulation of IL-2-dependent gammadelta T-cells with Con A induced IFN-gamma and IL-2 mRNA transcripts, whereas stimulation with rIL-2 induced only IFN-gamma mRNA. Subcutaneous injection of 3-week-old chickens with IL-2 DNA increased splenic cells, expressing the CD8 and gammadelta TCR antigens. To investigate the role of IL-2 and gammadelta T-cells in parasitic infection, chickens were orally infected with Eimeria acervulina and the expression of IL-2 mRNA transcripts in the spleen and duodenum and the percentage of gammadelta T-cells in the duodenum were examined. Following both, the primary and secondary infections, a significant enhancement of IL-2 mRNA transcripts in the spleen and intestine and increased percentage of intraepithelial gammadelta T-cells in the duodenum were observed. These results indicate that host immune responses to E. acervulina involve an up-regulation of IL-2 secretion and an increased duodenum gammadelta T-cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0165-2427
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
73
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
309-21
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10713343-Animals,
pubmed-meshheading:10713343-Chickens,
pubmed-meshheading:10713343-Coccidiosis,
pubmed-meshheading:10713343-Concanavalin A,
pubmed-meshheading:10713343-Cytotoxicity, Immunologic,
pubmed-meshheading:10713343-DNA Primers,
pubmed-meshheading:10713343-Duodenum,
pubmed-meshheading:10713343-Eimeria,
pubmed-meshheading:10713343-Flow Cytometry,
pubmed-meshheading:10713343-Interferon-gamma,
pubmed-meshheading:10713343-Interleukin-2,
pubmed-meshheading:10713343-Killer Cells, Natural,
pubmed-meshheading:10713343-Lymphocyte Activation,
pubmed-meshheading:10713343-Polymerase Chain Reaction,
pubmed-meshheading:10713343-Poultry Diseases,
pubmed-meshheading:10713343-RNA, Messenger,
pubmed-meshheading:10713343-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:10713343-Recombinant Proteins,
pubmed-meshheading:10713343-Spleen,
pubmed-meshheading:10713343-T-Lymphocytes,
pubmed-meshheading:10713343-Up-Regulation
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| pubmed:year |
2000
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| pubmed:articleTitle |
Role of chicken IL-2 on gammadelta T-cells and Eimeria acervulina-induced changes in intestinal IL-2 mRNA expression and gammadelta T-cells.
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| pubmed:affiliation |
Immunology and Disease Resistance Laboratory, Livestock and Poultry Sciences Institute, Building 1040, BARC-East, USDA, Beltsville, MD 207O5, USA.
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| pubmed:publicationType |
Journal Article
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