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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-4-10
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pubmed:abstractText |
The present study investigated whether an explanation for the conflicting reports on the interleukin-2 (IL-2) status of amniotic fluid is due to the presence of IL-15 which shares biological activities with IL-2 and utilizes the IL-2 receptor beta-chain. Amniotic fluids from 45 normally progressing pregnancies between 14 and 16 weeks after the last menstrual period were assayed for IL-2 and IL-15 by bioassay and enzyme-linked immunosorbent assay (ELISA). The ability of amniotic fluids to induce cytotoxic T lymphoblastoid line-2 (CTLL-2) cell proliferation was demonstrated to be dependent upon bioassay culture conditions. In serum-free medium each amniotic fluid stimulated CTLL-2 proliferation with a mean level of IL-2-like bioactivity of 14.7 +/- 2.3 ng/ml but amniotic fluids failed to induce CTLL-2 proliferation in serum-supplemented medium. Treatment with neutralizing anti-IL-2 or anti-IL-15 antibodies failed to inhibit amniotic fluid-induced CTLL cell proliferation in serum-free medium, indicating a lack of IL-2 and IL-15 bioactivity. In contrast, treatment with anti-IL-2 receptor beta-chain antibody significantly reduced amniotic fluid-induced proliferation. The lack of IL-2 and IL-15 activity in amniotic fluids was confirmed using ELISA. Although high levels of IL-15 immunoactivity were detected in all samples, specificity controls showed a lack of specific IL-15 immunoactivity in amniotic fluid. Pretreatment of amniotic fluids with 100-500 ng/ml mouse immunoglobulin G abrogated IL-15 immunoactivity, indicating that amniotic fluid contains molecules binding to Fc regions of immunoglobulins and responsible for false ELISA positivity. These studies unequivocally show that amniotic fluid lacks IL-2 and IL-15 but can stimulate CTLL-2 cell proliferation via the IL-2 receptor beta-chain. The absence of IL-2 and IL-15 in normal mid-trimester amniotic fluid suggests that the cytokine profile of human pregnancy appears to be associated with a bias against type 1 cytokines within the feto-placental unit.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-10203701,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-1696527,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-1888885,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-2119428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-2362563,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-2786341,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-2786842,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-7485325,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-7498714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-7499878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-7518368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-7523571,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-7525703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-7574904,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-7683506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-7814861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-7821485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-7892891,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-7929746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8026467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8178155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8180676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8362955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8363725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8574962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8589267,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8636925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8639878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8675621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8698270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8874712,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8953522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-8960615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-9023815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-9058793,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-9120746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-9357139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712671-9458929
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0019-2805
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
411-7
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10712671-Amniotic Fluid,
pubmed-meshheading:10712671-Antibodies, Blocking,
pubmed-meshheading:10712671-Biological Assay,
pubmed-meshheading:10712671-Cytotoxicity Tests, Immunologic,
pubmed-meshheading:10712671-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10712671-Female,
pubmed-meshheading:10712671-Humans,
pubmed-meshheading:10712671-Interleukin-15,
pubmed-meshheading:10712671-Interleukin-2,
pubmed-meshheading:10712671-Interleukins,
pubmed-meshheading:10712671-Lymphocyte Activation,
pubmed-meshheading:10712671-Pregnancy,
pubmed-meshheading:10712671-Pregnancy Trimester, Second,
pubmed-meshheading:10712671-Receptors, Interleukin-2
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pubmed:year |
2000
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pubmed:articleTitle |
Human amniotic fluid lacks interleukin-2 and interleukin-15 but can interact with the beta-chain of the interleukin-2 receptor.
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pubmed:affiliation |
Department of Microbiology and Immunology, The Medical School, University of Newcastle upon Tyne, UK.
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pubmed:publicationType |
Journal Article
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