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rdf:type |
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lifeskim:mentions |
umls-concept:C0004409,
umls-concept:C0007589,
umls-concept:C0010815,
umls-concept:C0019932,
umls-concept:C0037083,
umls-concept:C0162741,
umls-concept:C0205349,
umls-concept:C0456205,
umls-concept:C0596988,
umls-concept:C1511938,
umls-concept:C1548328,
umls-concept:C1550605,
umls-concept:C1553412,
umls-concept:C1710082,
umls-concept:C1720899
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pubmed:issue |
3
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pubmed:dateCreated |
2000-5-25
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pubmed:abstractText |
We describe mutant tissue lines of Arabidopsis that are able to grow in vitro as callus on hormone-free medium. The 14 lines presented here show different hormone autotrophic differentiation behaviors that can be classified into three categories: (a) forming roots (rooty callus), (b) forming shoots or shoot-like structures (shooty callus), or (c) growing without organ formation (callus). Three fast-growing lines showed altered steady-state mRNA levels of the Cdc2 and CycD3 cell cycle genes. Three of the six rooty callus lines contained about 20- to 30-fold higher levels of auxins than wild-type callus. These and two other lines with normal auxin content showed an increased steady-state level of IAA1 and IAA2 transcripts in the absence of exogenous auxin. Five of the six shooty callus lines had increased steady-state mRNA levels of the CKI1 gene and/or of the homeobox genes KNAT1 and STM, suggesting that the phenotype is linked to altered cytokinin signaling. Also, one cytokinin-overproducing line with only 5% of wild-type cytokinin oxidase activity was identified. These results indicate that screening for hormone-autonomous growth identifies mutants with altered hormone content or signaling.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-10049708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-10066178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-10230061,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-10417706,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-13486467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-14239480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-16653233,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-16664650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-16666783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-16668373,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-16668504,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-2468132,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-7657165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-7658471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-7664733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-7684007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-7849758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-7866029,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-8305869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-8453274,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-8538741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-8589625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-8606769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-8612271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-8776897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-8875940,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-9011081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-9039260,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-9203344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-9301091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-9574502,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-9675903,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10712535-9761792
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0032-0889
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
122
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
721-9
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pubmed:dateRevised |
2010-9-13
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pubmed:meshHeading |
pubmed-meshheading:10712535-Arabidopsis,
pubmed-meshheading:10712535-Cell Cycle,
pubmed-meshheading:10712535-Cytokinins,
pubmed-meshheading:10712535-Indoleacetic Acids,
pubmed-meshheading:10712535-Mutation,
pubmed-meshheading:10712535-Phenotype,
pubmed-meshheading:10712535-Plant Growth Regulators,
pubmed-meshheading:10712535-RNA, Messenger,
pubmed-meshheading:10712535-RNA, Plant,
pubmed-meshheading:10712535-Signal Transduction
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pubmed:year |
2000
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pubmed:articleTitle |
Hormone autotrophic growth and differentiation identifies mutant lines of Arabidopsis with altered cytokinin and auxin content or signaling.
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pubmed:affiliation |
Universität Tübingen, Center for Plant Molecular Biology (ZMBP), Allgemeine Genetik, Auf der Morgenstelle 28, D-72076 Tübingen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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