Linked Life Data

10706728

Source:http://linkedlifedata.com/resource/pubmed/id/10706728

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-3-28
pubmed:abstractText
Rats immunized with Mycobacterium butyricum in Freund's adjuvant develop a chronic vasculitis, with large increases in leukocyte rolling and adhesion in mesenteric postcapillary venules that are significantly inhibited with an alpha 4 integrin Ab. Using intravital microscopy to visualize chronically inflamed microvessels, we demonstrated that alpha 4 integrin-dependent leukocyte rolling and adhesion was inhibited with a beta 1 integrin, but not a beta 7 integrin Ab. To date, VCAM-1 has been presumed to be the primary ligand for alpha 4 beta 1 integrin in the vasculature. However, alpha 4 beta 1 integrin-dependent interactions were not reduced by monoclonal or polyclonal VCAM-1 Abs or a VCAM-1 antisense oligonucleotide despite increased VCAM-1 expression in the mesenteric vasculature. To ensure that the VCAM-1 Abs were functional and used at saturating concentrations, blood from Ab-treated rats was perfused over monolayers of CHO cells transfected with rat VCAM-1. Sufficient alpha 4 integrin or VCAM-1 Ab was present to inhibit leukocyte interactions with rat VCAM-1 by 95-100%. Under in vitro flow conditions, only mononuclear leukocytes were recruited from blood of control rats onto purified VCAM-1. However, neutrophils were also recruited onto VCAM-1 from whole blood of adjuvant-immunized animals via alpha 4 integrin. Another ligand for alpha 4 beta 1 integrin is the connecting segment-1 (CS-1) region of fibronectin. An Ab to the CS-1 portion of fibronectin, which did not reduce rolling and adhesion in adjuvant arthritis animals, completely inhibited leukocyte adhesion to CS-1 under static conditions. These findings provide the first evidence that alpha 4 beta 1 integrin-dependent leukocyte rolling and adhesion can occur in vivo via a mechanism other than VCAM-1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
164
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3337-44
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10706728-Adjuvants, Immunologic, pubmed-meshheading:10706728-Animals, pubmed-meshheading:10706728-Antibodies, Monoclonal, pubmed-meshheading:10706728-Antigens, CD, pubmed-meshheading:10706728-Arthritis, Experimental, pubmed-meshheading:10706728-Cell Movement, pubmed-meshheading:10706728-Chronic Disease, pubmed-meshheading:10706728-Cricetinae, pubmed-meshheading:10706728-Fibronectins, pubmed-meshheading:10706728-Injections, Intravenous, pubmed-meshheading:10706728-Integrin alpha4, pubmed-meshheading:10706728-Integrin alpha4beta1, pubmed-meshheading:10706728-Integrins, pubmed-meshheading:10706728-Leukocytes, pubmed-meshheading:10706728-Ligands, pubmed-meshheading:10706728-Male, pubmed-meshheading:10706728-Microcirculation, pubmed-meshheading:10706728-Neutrophils, pubmed-meshheading:10706728-Peptide Fragments, pubmed-meshheading:10706728-Rats, pubmed-meshheading:10706728-Rats, Sprague-Dawley, pubmed-meshheading:10706728-Receptors, Lymphocyte Homing, pubmed-meshheading:10706728-Vascular Cell Adhesion Molecule-1
pubmed:year
2000
pubmed:articleTitle
Alpha 4 integrin-dependent leukocyte recruitment does not require VCAM-1 in a chronic model of inflammation.
pubmed:affiliation
Immunology Research Group, Department of Physiology and Biophysics, University of Calgary, Calgary, Alberta, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't