Linked Life Data

10704713

Source:http://linkedlifedata.com/resource/pubmed/id/10704713

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-4-24
pubmed:abstractText
Aspergillus fumigatus, a ubiquitous fungus, is implicated in the pathogenesis of a number of clinically different allergic diseases in man. Peptide-based immunotherapy may offer an alternative in patient care and management. The purpose of this study was to evaluate the role of T cell epitopes of A. fumigatus ribotoxin, Asp f 1 in inducing tolerance in mice exposed to A. fumigatus antigen. The epitope analysis in BALB/c mice using synthetic peptides of Asp f 1 demonstrated both cryptic and dominant epitopes detected from 42 through 54 and 155 through 167 aa, accordingly. Intravenous injection of these peptides markedly inhibited the response induced by the exposure to crude A. fumigatus extract in mice as evidenced by the in vitro interleukin-2 (IL-2) production and proliferation of T-lymphocytes. Cytokine transcription studies indicate that, when stimulated with the peptides in immunogenic conditions, the major peptide (aa 155-167) specific T cell clone produced only IFN-gamma, but not IL-4. The ability of both dominant and cryptic peptide epitopes of a single molecule to induce tolerance against the immune response to a multi-molecular allergen complex has significant implication for peptide-based immunotherapy.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0196-9781
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-8
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:10704713-Allergens, pubmed-meshheading:10704713-Amino Acid Sequence, pubmed-meshheading:10704713-Animals, pubmed-meshheading:10704713-Antibodies, Fungal, pubmed-meshheading:10704713-Antigens, Fungal, pubmed-meshheading:10704713-Antigens, Plant, pubmed-meshheading:10704713-Aspergillus fumigatus, pubmed-meshheading:10704713-Cytokines, pubmed-meshheading:10704713-Epitopes, pubmed-meshheading:10704713-Fungal Proteins, pubmed-meshheading:10704713-Humans, pubmed-meshheading:10704713-Immune Tolerance, pubmed-meshheading:10704713-Immunoglobulin G, pubmed-meshheading:10704713-Injections, Intravenous, pubmed-meshheading:10704713-Lymphocyte Activation, pubmed-meshheading:10704713-Mice, pubmed-meshheading:10704713-Mice, Inbred BALB C, pubmed-meshheading:10704713-Molecular Sequence Data, pubmed-meshheading:10704713-RNA, Messenger, pubmed-meshheading:10704713-T-Lymphocytes
pubmed:year
2000
pubmed:articleTitle
Intravenous injection of major and cryptic peptide epitopes of ribotoxin, Asp f 1 inhibits T cell response induced by crude Aspergillus fumigatus antigens in mice.
pubmed:affiliation
Department of Immunology, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, RAS, Miklukho-Maklaya str, 16/10, 117871, Moscow, Russia. esvir@ibch.siobc.ras.ru
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't