Linked Life Data

10704676

Source:http://linkedlifedata.com/resource/pubmed/id/10704676

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-3-17
pubmed:abstractText
The immunoglobulin (Ig) genes are frequently involved in chromosomal rearrangements with a wide variety of partner loci in multiple myeloma (MM). However, several partner chromosomes have not been detected by conventional cytogenetic methods; for example, 4p16.3 (FGFR3), 6p25.3 (IRF4), and 16q23 (c-maf). To clarify the incidence of t(4;14)(p16.3;q32.3) in primary tumors of MM and to evaluate possible correlations with specific manifestations of the disease, G-banding, double-color fluorescence in situ hybridization (DC-FISH), and/or reverse-transcriptase polymerase chain reaction (RT-PCR) were performed on 40 patients with MM-two with plasmacytoma (PCM) and three with plasma cell leukemia (PCL). All patients were studied by DC-FISH; 40 were studied by G-banding and 36 were studied by RT-PCR. The FISH probes consisted of a cosmid pC385.12 containing the FGFR3 gene, a YAC Y6 containing VH, and a phage Iggamma1-10 containing the gamma1 constant region (Cgamma). We identified eight patients with either FGFR3/Cgamma fusion or FGFR3 overexpression: six patients with both FGFR3/Cgamma fusion and FGFR3 overexpression, one patient with FGFR3/Cgamma, and one with FGFR3 overexpression. FGFR3/Cgamma fusion was demonstrated at a frequency of 19% to 38% on interphase nuclei in seven of the 45 patients. Lytic bone lesions were found to be associated with FGFR3 overexpression. Interphase FISH with FGFR3 and Cgamma probes combined with RT-PCR proved to be an effective tool for detection of this fully cryptic translocation, thus facilitating the characterization of clinical features of MM patients with t(4;14).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0165-4608
pubmed:author
pubmed:issnType
Print
pubmed:volume
117
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
89-96
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10704676-Adult, pubmed-meshheading:10704676-Aged, pubmed-meshheading:10704676-Aged, 80 and over, pubmed-meshheading:10704676-Chromosome Banding, pubmed-meshheading:10704676-Chromosomes, Human, Pair 14, pubmed-meshheading:10704676-Chromosomes, Human, Pair 4, pubmed-meshheading:10704676-Female, pubmed-meshheading:10704676-Gene Expression, pubmed-meshheading:10704676-Humans, pubmed-meshheading:10704676-Immunoglobulin Heavy Chains, pubmed-meshheading:10704676-In Situ Hybridization, Fluorescence, pubmed-meshheading:10704676-Interphase, pubmed-meshheading:10704676-Karyotyping, pubmed-meshheading:10704676-Leukemia, Plasma Cell, pubmed-meshheading:10704676-Male, pubmed-meshheading:10704676-Middle Aged, pubmed-meshheading:10704676-Multiple Myeloma, pubmed-meshheading:10704676-Plasmacytoma, pubmed-meshheading:10704676-Protein-Tyrosine Kinases, pubmed-meshheading:10704676-Receptor, Fibroblast Growth Factor, Type 3, pubmed-meshheading:10704676-Receptors, Fibroblast Growth Factor, pubmed-meshheading:10704676-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:10704676-Translocation, Genetic
pubmed:year
2000
pubmed:articleTitle
Interphase detection of t(4;14)(p16.3;q32.3) by in situ hybridization and FGFR3 overexpression in plasma cell malignancies.
pubmed:affiliation
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Kyoto, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't