|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
2000-7-27
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
We recently cloned a novel murine transcriptional repressor, the Krüppel-like zinc finger protein AP-2rep (HGMW-approved symbol KLF12), that binds to a regulatory element in the AP-2alpha gene promoter. In the present study, we characterize the human AP-2rep homolog and describe expression patterns in human urogenital and lymphoma cell lines. The predicted human protein of 402 amino acids exhibits 95.8% identity and 98.5% similarity to the murine AP-2rep peptide. The genomic locus of human AP-2rep consists of seven exons and was assigned to chromosome 13q22 by fluorescence in situ hybridization to metaphase chromosomes. Human AP-2rep repressed both reporter expression from a transiently transfected AP-2alpha promoter and the endogenous AP-2alpha gene and inversely was negatively regulated by AP-2alpha. The consensus motif CAGTGGG was identified by an in vitro binding site selection assay. In summary, our data further point to an important role of AP-2rep as a transcriptional silencer and reveal reciprocal regulation of AP-2alpha and AP-2rep.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0888-7543
|
|
pubmed:author |
|
|
pubmed:copyrightInfo |
Copyright 2000 Academic Press.
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
63
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
384-90
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:10704285-Amino Acid Sequence,
pubmed-meshheading:10704285-Animals,
pubmed-meshheading:10704285-Burkitt Lymphoma,
pubmed-meshheading:10704285-Cells, Cultured,
pubmed-meshheading:10704285-Cercopithecus aethiops,
pubmed-meshheading:10704285-Chromosomes, Human, Pair 13,
pubmed-meshheading:10704285-Consensus Sequence,
pubmed-meshheading:10704285-DNA,
pubmed-meshheading:10704285-DNA, Complementary,
pubmed-meshheading:10704285-DNA-Binding Proteins,
pubmed-meshheading:10704285-Gene Expression,
pubmed-meshheading:10704285-Gene Silencing,
pubmed-meshheading:10704285-Genes,
pubmed-meshheading:10704285-HeLa Cells,
pubmed-meshheading:10704285-Humans,
pubmed-meshheading:10704285-In Situ Hybridization, Fluorescence,
pubmed-meshheading:10704285-Jurkat Cells,
pubmed-meshheading:10704285-Kidney,
pubmed-meshheading:10704285-Kidney Neoplasms,
pubmed-meshheading:10704285-Kruppel-Like Transcription Factors,
pubmed-meshheading:10704285-Male,
pubmed-meshheading:10704285-Mice,
pubmed-meshheading:10704285-Molecular Sequence Data,
pubmed-meshheading:10704285-Neoplasm Proteins,
pubmed-meshheading:10704285-Promoter Regions, Genetic,
pubmed-meshheading:10704285-Prostatic Neoplasms,
pubmed-meshheading:10704285-Recombinant Fusion Proteins,
pubmed-meshheading:10704285-Sequence Alignment,
pubmed-meshheading:10704285-Sequence Homology, Amino Acid,
pubmed-meshheading:10704285-Species Specificity,
pubmed-meshheading:10704285-Teratocarcinoma,
pubmed-meshheading:10704285-Transcription Factors,
pubmed-meshheading:10704285-Transfection,
pubmed-meshheading:10704285-Tumor Cells, Cultured
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
Genomic structure and DNA binding properties of the human zinc finger transcriptional repressor AP-2rep (KLF12).
|
|
pubmed:affiliation |
Institute for Pathology, University of Regensburg Medical School, Franz-Josef-Strauss-Allee, Regensburg, D-93042, Germany.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study
|
