Linked Life Data

10703721

Source:http://linkedlifedata.com/resource/pubmed/id/10703721

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2000-3-24
pubmed:abstractText
In the process of developing compounds to counteract the damaging effects of free radicals in biological systems it is important to determine the type and the intracellular location of specific toxic events. For that purpose cultured cells are used, because a properly designed cell culture system allows to asses specific damage at the cellular and subcellular level and can contribute to an evaluation of the biochemistry of free radical damage. A wide range of changes in cellular activities resulting from oxidative injury in vitro have been demonstrated. Data from many laboratories indicate that cell culture system coupled with appropriate analytical techniques can be used to explore cellular and biochemical details of damage induced by free radicals. The type of reactive oxygen species used to generate the radicals, the rate of radical production, and the location of action of the toxic species must be taken into account to understand the biochemistry of the system. An effort is made to analyse the considerable progress made in the development of appropriate in vitro models and end-points for use in testing and characterising the nature and location of free radical cytotoxicity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0231-5882
pubmed:author
pubmed:issnType
Print
pubmed:volume
18 Spec No
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
63-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
The use of cell culture systems for the assessment of general cellular toxicity and to detect the nature and location of free radical damage.
pubmed:affiliation
Department of Biochemistry and Microbiology, Faculty of Chemical Technology, Slovak University of Technology, Bratislava. horakova@chtf.stuba.sk
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't