Linked Life Data

10701499

Source:http://linkedlifedata.com/resource/pubmed/id/10701499

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-3-30
pubmed:abstractText
We report the average insulin response to acute glucose measured by in vitro perifusion of pancreatic islets isolated from 80 consecutive human organs. Different perifusion parameters were considered [basal release, stimulation index (SI), time to peak, incremental area under the curve delta-AUC alpha)], and the correlation among them was determined. SI positively correlated with delta-AUC alpha (p < 0.001, r = 0.80) while negatively with time to peak (p < 0.05, r = -0.23). We also evaluated several variables of the isolation procedure that might affect responsiveness to glucose by human islets. Sex and age of pancreas donors, cold ischemia time, duration of the digestion, collagenase concentration, and lot characteristics (collagenase, trypsin, clostripain, and proteases activity), and final islet yield were considered. Multivariate regression analysis showed only an independent association between SI and the concentration of collagenase (p = 0.01).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0963-6897
pubmed:author
pubmed:issnType
Print
pubmed:volume
8
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
709-12
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Lessons from in vitro perifusion of pancreatic islets isolated from 80 human pancreases.
pubmed:affiliation
Istituto Scientifico San Raffaele, Department of Surgery, University of Milan, Italy. bertuzzi.federico@hsr.it
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't