Linked Life Data

10700463

Source:http://linkedlifedata.com/resource/pubmed/id/10700463

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-5-1
pubmed:abstractText
Most human adults carry the Epstein-Barr virus (EBV) and develop immunological memory against the structural and the virus-encoded cellular proteins. The EBV nuclear antigen 6 (EBNA6) elicits cytotoxic T cell responses and it also maintains a persistent antibody response. The majority of sera from EBV-seropositive individuals reacts with a synthetic peptide, p63, comprising 21 amino acids of a repetitive region of EBNA6. CD4(+) T lymphocytes, with specificity for p63, could be recalled from the T cell repertoire of EBV carriers that expressed certain HLA-DR allotypes which were identified as good binders of p63 by an in vitro flow cytometric assay. Analysis of the HLA-DR/p63 interaction by molecular mechanics calculations indicated the presence of multiple overlapping epitopes which were predicted to bind in a HLA-DRB1 allo- and subtype-specific manner. Specific activation of p63-selected long-term CD4(+) T cell cultures resulted in a proliferative response, in the production of IL-2 and in the secretion of high levels of tumor necrosis factor as measured by bioassays. Proliferation and cytokine production of p63-specific T cells could be induced by p63-loaded HLA-DR-matched antigen-presenting cells and by B cells co-expressing relevant HLA-DR molecules and EBNA6. Our results show that peptides of an EBNA6 repeat region induce CD4(+) T cells which can react with EBNA6-carrying cells in many individuals. We suggest that these T(h) cells may be important in conditioning dendritic cells for initiation potent virus-specific immune responses, provide help for EBV-specific B cells, drive IgG isotype switch and support the sustained effector function of memory cytotoxic T lymphocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0953-8178
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-93
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:10700463-Adult, pubmed-meshheading:10700463-Amino Acid Sequence, pubmed-meshheading:10700463-Animals, pubmed-meshheading:10700463-Biotinylation, pubmed-meshheading:10700463-CD4-Positive T-Lymphocytes, pubmed-meshheading:10700463-Carrier State, pubmed-meshheading:10700463-Cells, Cultured, pubmed-meshheading:10700463-DNA, Viral, pubmed-meshheading:10700463-Dendritic Cells, pubmed-meshheading:10700463-Epitopes, pubmed-meshheading:10700463-Epstein-Barr Virus Infections, pubmed-meshheading:10700463-Epstein-Barr Virus Nuclear Antigens, pubmed-meshheading:10700463-HLA-DR Antigens, pubmed-meshheading:10700463-HLA-DR alpha-Chains, pubmed-meshheading:10700463-HLA-DRB1 Chains, pubmed-meshheading:10700463-Herpesvirus 4, Human, pubmed-meshheading:10700463-Humans, pubmed-meshheading:10700463-Immunoglobulin Class Switching, pubmed-meshheading:10700463-Immunoglobulin G, pubmed-meshheading:10700463-Immunologic Memory, pubmed-meshheading:10700463-L Cells (Cell Line), pubmed-meshheading:10700463-Lymphocyte Activation, pubmed-meshheading:10700463-Lymphokines, pubmed-meshheading:10700463-Mice, pubmed-meshheading:10700463-Molecular Sequence Data, pubmed-meshheading:10700463-Peptide Fragments, pubmed-meshheading:10700463-Repetitive Sequences, Amino Acid, pubmed-meshheading:10700463-Transfection
pubmed:year
2000
pubmed:articleTitle
A repetitive sequence of Epstein-Barr virus nuclear antigen 6 comprises overlapping T cell epitopes which induce HLA-DR-restricted CD4(+) T lymphocytes.
pubmed:affiliation
Microbiology and Tumorbiology Center, Karolinska Institute, 17 177 Stockholm, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't