|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2000-4-21
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
We report a method for typing polymorphisms at the T-cell epitopes within the Th2R and Th3R regions of the Plasmodium falciparum circumsporozoite protein (CSP). This method combines the use of PCR and sequence specific oligonucleotide probes (PCR-SSOP), and allows the identification of single nucleotide polymorphisms in these epitope regions. PCR-SSOP is a robust and a high-throughput sequence typing technique which has the same specificity and fidelity as direct sequencing. This method has been developed specifically for the assessment of the protective efficacy of RTS,S/SBAS2 vaccine against the 3D7 strain of P. falciparum (RTS,S/SBAS2 vaccine contains a part of the 3D7 CSP protein) in a phase IIb trial in Gambia which has been completed recently. PCR-SSOP could be used to determine the allelic frequencies of other parasite antigens and their geographical distribution.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0166-6851
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
5
|
|
pubmed:volume |
106
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
273-82
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:10699256-Alleles,
pubmed-meshheading:10699256-Amino Acid Sequence,
pubmed-meshheading:10699256-Animals,
pubmed-meshheading:10699256-Antigens, Protozoan,
pubmed-meshheading:10699256-Base Sequence,
pubmed-meshheading:10699256-DNA, Protozoan,
pubmed-meshheading:10699256-DNA Primers,
pubmed-meshheading:10699256-Epitopes,
pubmed-meshheading:10699256-Gene Frequency,
pubmed-meshheading:10699256-Humans,
pubmed-meshheading:10699256-Malaria, Falciparum,
pubmed-meshheading:10699256-Molecular Sequence Data,
pubmed-meshheading:10699256-Oligonucleotide Probes,
pubmed-meshheading:10699256-Plasmodium falciparum,
pubmed-meshheading:10699256-Polymerase Chain Reaction,
pubmed-meshheading:10699256-Polymorphism, Genetic,
pubmed-meshheading:10699256-Protozoan Proteins,
pubmed-meshheading:10699256-T-Lymphocytes
|
|
pubmed:year |
2000
|
|
pubmed:articleTitle |
High-throughput sequence typing of T-cell epitope polymorphisms in Plasmodium falciparum circumsporozoite protein.
|
|
pubmed:affiliation |
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, UK. ali.alloueche@lshtm.ac.uk
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
