Linked Life Data

10698965

Source:http://linkedlifedata.com/resource/pubmed/id/10698965

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2000-4-10
pubmed:abstractText
Matrix metalloproteinases (MMPs) are a family of Zn(2+)-dependent extracellular proteases capable of degrading various proteinaceous components of the extracellular matrix (ECM). They are expressed in developmental and pathological processes such as postlactation mammary gland involution and tumor metastasis. Relatively few studies have been carried out to investigate the function of MMPs during embryogenesis and postembryonic organ development. Using Xenopus development as a model system, we and others have previously isolated three MMP genes as thyroid hormone response genes. They have distinct temporal and organ-specific regulations during thyroid hormone-dependent metamorphosis. We demonstrate here that three MMPs-stromelysin-3 (ST3), collagenases-3 (Col3), and collagenases-4 (Col4)-also have distinct spatial and temporal expression profiles during embryogenesis. Consistent with earlier suggestions that ST3 is a direct thyroid hormone response gene whereas Col3 and Col4 are not, we show that precocious overexpression of thyroid hormone receptors in the presence of thyroid hormone lead to increased expression of ST3, but not Col3. Furthermore, our whole-mount in situ hybridizations reveal a tight but distinct association of individual MMPs with tissue remodeling in different regions of the animal during embryogenesis. These results suggest that ST3 is likely to play a role in ECM remodeling that facilitate apoptotic tissue remodeling or resorption, whereas Col3 and Col4 appear to participate in connective tissue degradation during development.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0892-6638
pubmed:author
pubmed:issnType
Print
pubmed:volume
14
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
503-10
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10698965-Animals, pubmed-meshheading:10698965-Collagenases, pubmed-meshheading:10698965-Embryo, Nonmammalian, pubmed-meshheading:10698965-Female, pubmed-meshheading:10698965-Gene Expression Regulation, Developmental, pubmed-meshheading:10698965-Gene Expression Regulation, Enzymologic, pubmed-meshheading:10698965-In Situ Hybridization, pubmed-meshheading:10698965-Matrix Metalloproteinase 11, pubmed-meshheading:10698965-Matrix Metalloproteinase 13, pubmed-meshheading:10698965-Metalloendopeptidases, pubmed-meshheading:10698965-Oocytes, pubmed-meshheading:10698965-RNA, Messenger, pubmed-meshheading:10698965-Receptors, Retinoic Acid, pubmed-meshheading:10698965-Receptors, Thyroid Hormone, pubmed-meshheading:10698965-Retinoid X Receptors, pubmed-meshheading:10698965-Transcription, Genetic, pubmed-meshheading:10698965-Transcription Factors, pubmed-meshheading:10698965-Triiodothyronine, pubmed-meshheading:10698965-Xenopus laevis
pubmed:year
2000
pubmed:articleTitle
Differential regulation of three thyroid hormone-responsive matrix metalloproteinase genes implicates distinct functions during frog embryogenesis.
pubmed:affiliation
Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, 20892, USA.
pubmed:publicationType
Journal Article