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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5458
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pubmed:dateCreated |
2000-3-23
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pubmed:abstractText |
A type III protein secretion system encoded by Salmonella pathogenicity island 2 (SPI2) has been found to be required for virulence and survival within macrophages. Here, SPI2 was shown to allow Salmonella typhimurium to avoid NADPH oxidase-dependent killing by macrophages. The ability of SPI2-mutant bacteria to survive in macrophages and to cause lethal infection in mice was restored by abrogation of the NADPH oxidase-dependent respiratory burst. Ultrastructural and immunofluorescence microscopy demonstrated efficient localization of the NADPH oxidase in the proximity of vacuoles containing SPI2-mutant but not wild-type bacteria, suggesting that SPI2 interferes with trafficking of oxidase-containing vesicles to the phagosome.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
287
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1655-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:10698741-Animals,
pubmed-meshheading:10698741-Bacterial Proteins,
pubmed-meshheading:10698741-Cerium,
pubmed-meshheading:10698741-Genes, Bacterial,
pubmed-meshheading:10698741-Hydroxides,
pubmed-meshheading:10698741-Macrophages, Peritoneal,
pubmed-meshheading:10698741-Mice,
pubmed-meshheading:10698741-Mice, Inbred C57BL,
pubmed-meshheading:10698741-Mice, Knockout,
pubmed-meshheading:10698741-Microscopy, Electron,
pubmed-meshheading:10698741-Microscopy, Fluorescence,
pubmed-meshheading:10698741-NADPH Oxidase,
pubmed-meshheading:10698741-Peroxides,
pubmed-meshheading:10698741-Phagosomes,
pubmed-meshheading:10698741-Respiratory Burst,
pubmed-meshheading:10698741-Salmonella Infections, Animal,
pubmed-meshheading:10698741-Salmonella typhimurium,
pubmed-meshheading:10698741-Superoxides,
pubmed-meshheading:10698741-Tetradecanoylphorbol Acetate,
pubmed-meshheading:10698741-Vacuoles,
pubmed-meshheading:10698741-Virulence
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pubmed:year |
2000
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pubmed:articleTitle |
Salmonella pathogenicity island 2-dependent evasion of the phagocyte NADPH oxidase.
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pubmed:affiliation |
Department of Medicine, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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