Source:http://linkedlifedata.com/resource/pubmed/id/10698073
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2000-3-9
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| pubmed:abstractText |
Dynamic changes in the regional cerebral glucose metabolic rate induced by hypoxia/reoxygenation or ischemia/reperfusion were investigated with a positron autoradiography technique. Fresh rat brain slices were incubated with [18F]2-fluoro-2-deoxy-D-glucose ([18F]FDG) in oxygenated Krebs-Ringer solution at 36 degrees C, and serial two-dimensional time-resolved images of [18F]FDG uptake in the slices were obtained. In the case of loading hypoxia (oxygen deprivation)/pseudoischemia (oxygen and glucose deprivation) for various periods of time, the net influx constant (K) of [18F]FDG at preloading and after reoxygenation/pseudoreperfusion (post-loading) was quantitatively evaluated by applying the Patlak graphical method to the image data. Regardless of the brain region, with hypoxia lasting > or =20 minutes, the postloading K value was decreased compared with the unloaded control, whereas with pseudoischemia of < or =40 minutes, approximately the same level as the unloaded control was maintained. Next, the neuroprotective effect against hypoxia/pseudoischemia loading induced by the addition of a free radical scavenger or an N-methyl-D-aspartate (NMDA) antagonist was assessed by determining whether a decrease in the postloading K value was prevented. Whereas with 20-minute hypoxia, both agents exhibited a neuroprotective effect, in the case of 50-minute pseudoischemia, only the NMDA antagonist did so, with the free radical scavenger being ineffective. These results demonstrate that hypoxia causes irreversible neuronal damage within a shorter period than ischemia, with both free radicals and glutamate suggested to be involved in tandem in the neurotoxicity induced by hypoxia, whereas glutamate alone is involved in ischemic neurotoxicity.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic N-Oxides,
http://linkedlifedata.com/resource/pubmed/chemical/Dizocilpine Maleate,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorodeoxyglucose F18,
http://linkedlifedata.com/resource/pubmed/chemical/Free Radicals,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotoxins,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrogen Oxides,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygen,
http://linkedlifedata.com/resource/pubmed/chemical/Spin Labels,
http://linkedlifedata.com/resource/pubmed/chemical/phenyl-N-tert-butylnitrone
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
0271-678X
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
20
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
350-8
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10698073-Animals,
pubmed-meshheading:10698073-Autoradiography,
pubmed-meshheading:10698073-Brain,
pubmed-meshheading:10698073-Cerebrovascular Circulation,
pubmed-meshheading:10698073-Cyclic N-Oxides,
pubmed-meshheading:10698073-Dizocilpine Maleate,
pubmed-meshheading:10698073-Excitatory Amino Acid Antagonists,
pubmed-meshheading:10698073-Fluorodeoxyglucose F18,
pubmed-meshheading:10698073-Free Radicals,
pubmed-meshheading:10698073-Glucose,
pubmed-meshheading:10698073-Glutamic Acid,
pubmed-meshheading:10698073-Hypoxia, Brain,
pubmed-meshheading:10698073-Ischemic Attack, Transient,
pubmed-meshheading:10698073-Male,
pubmed-meshheading:10698073-Neurotoxins,
pubmed-meshheading:10698073-Nitrogen Oxides,
pubmed-meshheading:10698073-Oxygen,
pubmed-meshheading:10698073-Rats,
pubmed-meshheading:10698073-Rats, Sprague-Dawley,
pubmed-meshheading:10698073-Reperfusion Injury,
pubmed-meshheading:10698073-Spin Labels
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| pubmed:year |
2000
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| pubmed:articleTitle |
Hypoxic but not ischemic neurotoxicity of free radicals revealed by dynamic changes in glucose metabolism of fresh rat brain slices on positron autoradiography.
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| pubmed:affiliation |
Biomedical Imaging Research Center, Fukui Medical University, Japan.
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| pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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