Linked Life Data

10695885

Source:http://linkedlifedata.com/resource/pubmed/id/10695885

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-3-21
pubmed:abstractText
In this study, plasma time-activity curves of 99mTc-mebrofenin were used to quantify hepatic function in dogs before and after induction of hepatic damage using a hepatotoxic agent. Nine dogs were determined to be healthy on the basis of physical examination, laboratory data and hepatic imaging. Plasma samples were collected 1, 3, 5, 7, 9, 15, 20, 30, 40, 50, and 60 minutes following a peripheral venous injection of 111-222 MBq (3-6 mCi) of 99mTc-mebrofenin. The area under the plasma time-activity curve (AUC) was calculated using two different methods and compared to direct measurement of the hepatic extraction efficiency. First pass hepatic extraction efficiency of 99mTc-mebrofenin was calculated from differential equation analysis of a two-compartment model following mesenteric venous injection of the radiopharmaceutical. In 7 of the original 9 dogs and 2 additional healthy dogs, plasma clearance and hepatic extraction efficiency determination were repeated following induction of hepatic injury by thiacetarsamide (3 mg/kg IV twice daily for 1 day). In one additional dog, hepatic injury was induced using carbon tetrachloride (0.3 ml/kg IP). Plasma time-activity curves of 99mTc-mebrofenin had kinetics of a two compartment model. Area under the curve was highly correlated with hepatic extraction efficiency. The AUC integrated from 1-60 minutes (AUC60) had the best correlation with hepatic extraction efficiency (r2 = 0.978, p < 0.001). A formula for calculation of hepatic extraction efficiency was derived using linear regression analysis: hepatic extraction efficiency = 105.583 - 3.099 x 10(5) x AUC60. Plasma clearance of a peripheral venous injection of 99mTc-mebrofenin is a simple, non-invasive, convenient method to quantify hepatic function which can be performed without a gamma camera.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1058-8183
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
78-84
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10695885-Algorithms, pubmed-meshheading:10695885-Animals, pubmed-meshheading:10695885-Area Under Curve, pubmed-meshheading:10695885-Arsenamide, pubmed-meshheading:10695885-Carbon Tetrachloride, pubmed-meshheading:10695885-Dog Diseases, pubmed-meshheading:10695885-Dogs, pubmed-meshheading:10695885-Drug-Induced Liver Injury, pubmed-meshheading:10695885-Filaricides, pubmed-meshheading:10695885-Follow-Up Studies, pubmed-meshheading:10695885-Imino Acids, pubmed-meshheading:10695885-Injections, Intravenous, pubmed-meshheading:10695885-Linear Models, pubmed-meshheading:10695885-Liver, pubmed-meshheading:10695885-Liver Diseases, pubmed-meshheading:10695885-Mesenteric Veins, pubmed-meshheading:10695885-Organotechnetium Compounds, pubmed-meshheading:10695885-Radiopharmaceuticals, pubmed-meshheading:10695885-Solvents
pubmed:articleTitle
Evaluation of plasma time-activity curves of technetium-99m-mebrofenin for measurement of hepatic function in dogs.
pubmed:affiliation
Department of Small Animal Clinical Sciences, University of Tennessee College of Veterinary Medicine, Knoxville 37901, USA.
pubmed:publicationType
Journal Article, Comparative Study