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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-4-21
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pubmed:abstractText |
We have previously reported that a fragment of mRNA, denoted as C7-1, which expression was significantly increased in the frontal cortex of aged rats. In the present study, we have cloned and sequenced the full length cDNA of the C7-1 gene. We have found that the open reading frame of this gene encoded a 463-amino-acid protein, which shared 84% identity in amino acid sequence with a subunit of vacuolar H(+)-ATPase (V-ATPase). Further Northern blot analysis revealed that there was an age-dependent increase in C7-1 gene expression in rat frontal cortex, but not in other brain areas. Moreover, application of C7-1 antisense oligonucleotide to cortical neuronal cultures markedly inhibited cell survival. These results together suggest that C7-1 is a marker for the aging process and that upregulation of C7-1 may be important in maintaining the normal function of V-ATPase during aging.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0169-328X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
75
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
330-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10686355-Aging,
pubmed-meshheading:10686355-Amino Acid Sequence,
pubmed-meshheading:10686355-Animals,
pubmed-meshheading:10686355-Base Sequence,
pubmed-meshheading:10686355-Biological Markers,
pubmed-meshheading:10686355-Blotting, Northern,
pubmed-meshheading:10686355-Cell Survival,
pubmed-meshheading:10686355-Cells, Cultured,
pubmed-meshheading:10686355-Cloning, Molecular,
pubmed-meshheading:10686355-DNA, Complementary,
pubmed-meshheading:10686355-Gene Expression Regulation,
pubmed-meshheading:10686355-Male,
pubmed-meshheading:10686355-Molecular Sequence Data,
pubmed-meshheading:10686355-Neurons,
pubmed-meshheading:10686355-Oligonucleotides, Antisense,
pubmed-meshheading:10686355-Prefrontal Cortex,
pubmed-meshheading:10686355-Protein Biosynthesis,
pubmed-meshheading:10686355-Proteins,
pubmed-meshheading:10686355-Proton-Translocating ATPases,
pubmed-meshheading:10686355-RNA, Messenger,
pubmed-meshheading:10686355-Rats,
pubmed-meshheading:10686355-Rats, Sprague-Dawley,
pubmed-meshheading:10686355-Sequence Homology, Amino Acid,
pubmed-meshheading:10686355-Tissue Distribution,
pubmed-meshheading:10686355-Vacuolar Proton-Translocating ATPases
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pubmed:year |
2000
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pubmed:articleTitle |
Age-dependent increase in C7-1 gene expression in rat frontal cortex.
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pubmed:affiliation |
Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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