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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2000-3-23
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pubmed:abstractText |
Recombinant human (rh) bone morphogenetic protein-2 (BMP-2) stimulates osteoblastic differentiation in cells isolated from human periodontal ligament (HPLC), and this action of rhBMP-2 may be modulated by prostaglandins (PGs), which are local regulatory factors in the bone metabolism. In the present study, we investigated the effect of prostaglandin E2 (PGE2) on rhBMP-2-stimulated osteoblastic differentiation in cultured HPLC. rhBMP-2 (500 ng/ml)-stimulated alkaline phosphatase (ALPase) activity was enhanced by simultaneous treatment with low concentrations (10(-10)-10(-8) M) of PGE2, whereas a high concentration (10(-6) M) of PGE2 suppressed it. rhBMP-2 did not induce cyclo-oxygenase-2 (COX-2) mRNA expression or subsequent PGE2 production, whereas it remarkably suppressed rhIL-1 beta-induced COX-2 mRNA expression and PGE2 production. The rhBMP-2 action on osteoblastic differentiation in HPLC was also enhanced by co-treatment with 0.25 to 25 ng/ml of rh interleukin-1 beta (IL-1 beta). The ALPase activity stimulated by simultaneous treatment with rhBMP-2 and rhIL-1 beta was partially inhibited by addition of 10(-6) M of indomethacin, which completely inhibited rhIL-1 beta-induced PGE2 production. These results reveal that PGE2 at different concentrations exerts a biphasic effect on BMP-2-stimulated osteoblastic differentiation in HPLC, BMP-2 inhibits IL-1 beta-induced PGE2 production through suppressing COX-2 expression, and the BMP-2-stimulated osteoblastic differentiation may be enhanced by the endogenous PGE2 induced by BMP-2 and IL-1 beta. These suggest that BMP-2 action on osteoblastic differentiation in HPLC may be modulated by PGE2 in autocrine and paracrine fashions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
D
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/BMP2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Protein 2,
http://linkedlifedata.com/resource/pubmed/chemical/Bone Morphogenetic Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase 2 Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclooxygenase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprostone,
http://linkedlifedata.com/resource/pubmed/chemical/Indomethacin,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/PTGS2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Peroxidases,
http://linkedlifedata.com/resource/pubmed/chemical/Prostaglandin-Endoperoxide Synthases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta,
http://linkedlifedata.com/resource/pubmed/chemical/recombinant human bone...
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0022-3484
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
34
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
431-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10685373-Adult,
pubmed-meshheading:10685373-Alkaline Phosphatase,
pubmed-meshheading:10685373-Autocrine Communication,
pubmed-meshheading:10685373-Bone Morphogenetic Protein 2,
pubmed-meshheading:10685373-Bone Morphogenetic Proteins,
pubmed-meshheading:10685373-Cell Differentiation,
pubmed-meshheading:10685373-Cells, Cultured,
pubmed-meshheading:10685373-Cyclooxygenase 2,
pubmed-meshheading:10685373-Cyclooxygenase 2 Inhibitors,
pubmed-meshheading:10685373-Cyclooxygenase Inhibitors,
pubmed-meshheading:10685373-Dinoprostone,
pubmed-meshheading:10685373-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:10685373-Humans,
pubmed-meshheading:10685373-Indomethacin,
pubmed-meshheading:10685373-Interleukin-1,
pubmed-meshheading:10685373-Isoenzymes,
pubmed-meshheading:10685373-Male,
pubmed-meshheading:10685373-Membrane Proteins,
pubmed-meshheading:10685373-Osteoblasts,
pubmed-meshheading:10685373-Paracrine Communication,
pubmed-meshheading:10685373-Periodontal Ligament,
pubmed-meshheading:10685373-Peroxidases,
pubmed-meshheading:10685373-Prostaglandin-Endoperoxide Synthases,
pubmed-meshheading:10685373-RNA, Messenger,
pubmed-meshheading:10685373-Recombinant Proteins,
pubmed-meshheading:10685373-Transforming Growth Factor beta
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pubmed:year |
1999
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pubmed:articleTitle |
Effect of prostaglandin E2 on recombinant human bone morphogenetic protein-2-stimulated osteoblastic differentiation in human periodontal ligament cells.
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pubmed:affiliation |
Department of Periodontics, Showa University Dental School, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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