Linked Life Data

10683175

Source:http://linkedlifedata.com/resource/pubmed/id/10683175

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2000-4-18
pubmed:abstractText
The Xenopus nodal related-1 (Xnr1) gene has a complex expression pattern in embryos, with two temporal phases. In the first phase, transcripts are first detected in perinuclear sites in the vegetal region of the blastula. During gastrulation, this expression disappears and transcripts become localised to the dorsal marginal zone. Expression stops and then restarts in a second phase at neurula and tailbud stages, firstly in two symmetric patches near the posterior end of the notochord, and then asymmetrically in a large domain in the left lateral plate mesoderm. In this study, we have investigated the regulation of the early phase of expression of Xnr1. We show that the T-box transcription factor VegT can induce Xnr1. It had previously been shown that Xnr1 can induce VegT in ectoderm cells and we show that the early expression of Xnr1 is regulated by an autoregulatory loop. By inspection of the Xnr1 promoter sequence, we have identified two non-palindromic T-box-binding sites, which are 10 bp apart. Using mutational analysis, we have shown that these elements are required for the VegT induction of Xnr1. The Xnr1 promoter shows striking homologies with the Xnr3 promoter. In particular, two elements that are required for Wnt signaling are conserved between these two promoters, but the two T-box sites are not conserved, and Xnr3 is not induced by VegT. A region of the promoter containing the T-box sites and the Wnt sites is sufficient to drive expression of a reporter gene in a dorsal domain in transgenic Xenopus at the gastrula stage. We show that this pattern of expression of the transgene in gastrulae is not dependent on the T-box sites.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0950-1991
pubmed:author
pubmed:issnType
Print
pubmed:volume
127
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1221-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10683175-Animals, pubmed-meshheading:10683175-Animals, Genetically Modified, pubmed-meshheading:10683175-Base Sequence, pubmed-meshheading:10683175-Conserved Sequence, pubmed-meshheading:10683175-DNA, Complementary, pubmed-meshheading:10683175-DNA Primers, pubmed-meshheading:10683175-Gastrula, pubmed-meshheading:10683175-Gene Expression Regulation, Developmental, pubmed-meshheading:10683175-Lac Operon, pubmed-meshheading:10683175-Molecular Sequence Data, pubmed-meshheading:10683175-Promoter Regions, Genetic, pubmed-meshheading:10683175-RNA, Messenger, pubmed-meshheading:10683175-Sequence Homology, Nucleic Acid, pubmed-meshheading:10683175-T-Box Domain Proteins, pubmed-meshheading:10683175-TATA Box, pubmed-meshheading:10683175-Transforming Growth Factor beta, pubmed-meshheading:10683175-Xenopus, pubmed-meshheading:10683175-Xenopus Proteins
pubmed:year
2000
pubmed:articleTitle
Regulation of the early expression of the Xenopus nodal-related 1 gene, Xnr1.
pubmed:affiliation
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't