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rdf:type |
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lifeskim:mentions |
umls-concept:C0007277,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0076252,
umls-concept:C0205266,
umls-concept:C0229571,
umls-concept:C0242184,
umls-concept:C0871261,
umls-concept:C1327616,
umls-concept:C1519355,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2911692
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pubmed:issue |
5
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pubmed:dateCreated |
2000-4-11
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pubmed:abstractText |
We have developed a thin-slice preparation of whole rat carotid body that allows us to perform patch-clamp recording of membrane ionic currents and to monitor catecholamine secretion by amperometry in single glomus cells under direct visual control. In normoxic conditions (P(O(2)) approximately 140 mmHg; 1 mmHg = 133 Pa), most glomus cells did not have measurable secretory activity, but exposure to hypoxia (P(O(2)) approximately 20 mmHg) elicited the appearance of a large number of spike-like exocytotic events. This neurosecretory response to hypoxia was fully reversible and required extracellular Ca(2+) influx. The average charge of single quantal events was 46 +/- 25 fC (n = 218), which yields an estimate of approximately 140,000 catecholamine molecules per vesicle. Addition of tetraethylammonium (TEA; 2-5 mM) to the extracellular solution induced in most (>95%) cells tested (n = 32) a secretory response similar to that elicited by low P(O(2)). Cells nonresponsive to hypoxia but activated by exposure to high external K(+) were also stimulated by TEA. A secretory response similar to the responses to hypoxia and TEA was also observed after treatment of the cells with iberiotoxin to block selectively Ca(2+)- and voltage-activated maxi-K(+) channels. Our data further show that membrane ion channels are critically involved in sensory transduction in the carotid body. We also show that in intact glomus cells inhibition of voltage-dependent K(+) channels can contribute to initiation of the secretory response to low P(O(2)).
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-1331289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-1354258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-1694175,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-1704113,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-2011601,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-2041730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-2544656,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-2737279,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-2780225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-2972065,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-3419588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-6270629,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-7539843,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-7896598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-7937863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-7938227,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-8021833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-8057251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-8741735,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-8888521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-9046009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-9051577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-9080372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-9174993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-9231723,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10681419-9630601
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
97
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2361-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
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pubmed:year |
2000
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pubmed:articleTitle |
Secretory responses of intact glomus cells in thin slices of rat carotid body to hypoxia and tetraethylammonium.
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pubmed:affiliation |
Departamento de Fisiologia, Facultad de Medicina y Hospital Universitario Virgen del Rocio, Universidad de Sevilla, E-41009, Sevilla, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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