Linked Life Data

10679930

Source:http://linkedlifedata.com/resource/pubmed/id/10679930

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-4-4
pubmed:abstractText
We have previously described a 5; cis-acting retinoic acid response element that is required for a subset of Hoxa4 expression, including the midgestation mouse lung. As both retinoids and Hox genes have been implicated in lung development and patterning, we have examined Hoxa4 expression in the developing mouse lung and extended our work on its regulation. At E12.5, a Hoxa4/lacZ transgene is expressed in the mesenchymal compartment of the lung. Later in development expression is restricted to the proximal mesenchyme and is also observed in smooth muscle cells, subepithelial fibroblasts, and alveolar cells. We show that both Hoxa4 and Hoxa5 are upregulated when cultured in the presence of all-trans retinoic acid. In addition, retinoic acid extends the domain of Hoxa4 and Hoxa5 expression to the periphery of the explants where the distal epithelia are developing. Interestingly, the effect of retinoic acid on Hoxa5 expression was not observed in a Hoxa4 mutant background. In contrast, TGFbeta1 was found to downregulate both Hoxa4 and Hoxa5 expression in cultured lung explants. We also establish that retinoic acid has the effect of proximalizing the mouse lung when cultured in a serum-free medium, as evidenced by reduced expression of the distal marker surfactant protein-C. Lungs from Hoxa4 mutant embryos exhibited a similar response to retinoic acid, suggesting that Hoxa4 alone is not required for the proximalizing effect. Based on their retinoid-dependent expression, we conclude that members of the group 4 and/or group 5 Hox genes are likely to be involved in patterning of the mouse lung. Dev Dyn 2000;217:62-74.
pubmed:grant
pubmed:keyword
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1058-8388
pubmed:author
pubmed:copyrightInfo
Copyright 2000 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
217
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
62-74
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
Regulation of the Hoxa4 and Hoxa5 genes in the embryonic mouse lung by retinoic acid and TGFbeta1: implications for lung development and patterning.
pubmed:affiliation
Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.