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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-3-22
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pubmed:abstractText |
We have shown that muramyl dipeptide (MDP) conjugated to a 10-mer polyguanylic acid (PolyG) is specifically internalized by macrophages through scavenger receptor (SCR)-mediated endocytosis. Macrophages activated by PolyG-MDP displayed about 20-fold higher cytotoxic activity against nonmacrophage tumor cells compared to that elicited by free MDP. The PolyG-MDP was found to trigger the secretion of higher levels of interleukin-6, interleukin-1alpha, TNF-alpha, and nitric oxide in comparison to free MDP. Addition of antibodies directed against IL-6 and TNF-alpha to macrophage culture completely abrogated the tumoricidal response of PolyG-MDP, indicating that these two cytokines are primarily responsible for bioefficacy. This general approach of PolyG as a vehicle may find wide application in the delivery of genes and antisense oligonucleotides to macrophages.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylmuramyl-Alanyl-Isoglutamine,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Poly G,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Scavenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 2000 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
268
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
772-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10679281-Acetylmuramyl-Alanyl-Isoglutamine,
pubmed-meshheading:10679281-Animals,
pubmed-meshheading:10679281-Binding Sites,
pubmed-meshheading:10679281-Cytotoxicity, Immunologic,
pubmed-meshheading:10679281-Drug Carriers,
pubmed-meshheading:10679281-Interleukin-1,
pubmed-meshheading:10679281-Interleukin-6,
pubmed-meshheading:10679281-Macrophage Activation,
pubmed-meshheading:10679281-Macrophages, Peritoneal,
pubmed-meshheading:10679281-Mice,
pubmed-meshheading:10679281-Mice, Inbred C57BL,
pubmed-meshheading:10679281-Nitric Oxide,
pubmed-meshheading:10679281-Poly G,
pubmed-meshheading:10679281-Receptors, Immunologic,
pubmed-meshheading:10679281-Receptors, Scavenger,
pubmed-meshheading:10679281-Tumor Cells, Cultured,
pubmed-meshheading:10679281-Tumor Necrosis Factor-alpha
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pubmed:year |
2000
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pubmed:articleTitle |
Selective activation of antitumor activity of macrophages by the delivery of muramyl dipeptide using a novel polynucleotide-based carrier recognized by scavenger receptors.
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pubmed:affiliation |
National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi, 110067, India.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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