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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5456
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pubmed:dateCreated |
2000-3-6
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pubmed:abstractText |
Steroid receptors bind to site-specific response elements in chromatin and modulate gene expression in a hormone-dependent fashion. With the use of a tandem array of mouse mammary tumor virus reporter elements and a form of glucocorticoid receptor labeled with green fluorescent protein, targeting of the receptor to response elements in live mouse cells was observed. Photobleaching experiments provide direct evidence that the hormone-occupied receptor undergoes rapid exchange between chromatin and the nucleoplasmic compartment. Thus, the interaction of regulatory proteins with target sites in chromatin is a more dynamic process than previously believed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0036-8075
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
287
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1262-5
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pubmed:dateRevised |
2007-3-19
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pubmed:meshHeading |
pubmed-meshheading:10678832-Animals,
pubmed-meshheading:10678832-Binding Sites,
pubmed-meshheading:10678832-Cell Line, Transformed,
pubmed-meshheading:10678832-Cell Nucleus,
pubmed-meshheading:10678832-Chromatin,
pubmed-meshheading:10678832-Dexamethasone,
pubmed-meshheading:10678832-Green Fluorescent Proteins,
pubmed-meshheading:10678832-In Situ Hybridization, Fluorescence,
pubmed-meshheading:10678832-Ligands,
pubmed-meshheading:10678832-Luminescent Proteins,
pubmed-meshheading:10678832-Mammary Tumor Virus, Mouse,
pubmed-meshheading:10678832-Mice,
pubmed-meshheading:10678832-Microscopy, Confocal,
pubmed-meshheading:10678832-Microscopy, Fluorescence,
pubmed-meshheading:10678832-Nucleosomes,
pubmed-meshheading:10678832-Receptors, Glucocorticoid,
pubmed-meshheading:10678832-Response Elements,
pubmed-meshheading:10678832-Terminal Repeat Sequences
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pubmed:year |
2000
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pubmed:articleTitle |
The glucocorticoid receptor: rapid exchange with regulatory sites in living cells.
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pubmed:affiliation |
Laboratory of Receptor Biology and Gene Expression, Building 41, Room B602, National Cancer Institute, Bethesda, MD 20892-5055, USA.
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pubmed:publicationType |
Journal Article
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