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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2000-2-29
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pubmed:abstractText |
Gene therapy is a novel therapeutic approach that might soon improve the prognosis of some cancers. We investigated the feasibility of cytosine deaminase (CD) suicide gene therapy in a model of peritoneal carcinomatosis. DHD/K12 colorectal adenocarcinoma cells transfected in vitro with the CD gene were highly sensitive to 5-fluorocytosine (5-FC), and a bystander effect could also be observed. Treating CD+ cells with 5-FC resulted in apoptosis as detected by terminal deoxynucleotidyltransferase-mediated deoxyuridine triphosphate nick-end labeling. In vitro, several human cell lines derived from ovarian or colorectal carcinomas, as well as the rat glioblastoma 9 L cell line, responded to CD/5-FC and showed a very strong bystander effect. 5-FC treatment of peritoneal carcinomatosis generated in syngeneic BDIX rats by CD-expressing DHD/K12 cells led to a complete and prolonged response and to prolonged survival. Our study thus demonstrated the efficacy of CD suicide gene therapy for the treatment of peritoneal carcinomatosis.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0929-1903
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
20-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10678352-Animals,
pubmed-meshheading:10678352-Antimetabolites, Antineoplastic,
pubmed-meshheading:10678352-Apoptosis,
pubmed-meshheading:10678352-Carcinoma,
pubmed-meshheading:10678352-Colorectal Neoplasms,
pubmed-meshheading:10678352-Cytosine Deaminase,
pubmed-meshheading:10678352-Disease Models, Animal,
pubmed-meshheading:10678352-Feasibility Studies,
pubmed-meshheading:10678352-Flucytosine,
pubmed-meshheading:10678352-Gene Therapy,
pubmed-meshheading:10678352-Humans,
pubmed-meshheading:10678352-Male,
pubmed-meshheading:10678352-Neoplasm Transplantation,
pubmed-meshheading:10678352-Nucleoside Deaminases,
pubmed-meshheading:10678352-Peritoneal Neoplasms,
pubmed-meshheading:10678352-Rats,
pubmed-meshheading:10678352-Tumor Cells, Cultured,
pubmed-meshheading:10678352-Uridine
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pubmed:year |
2000
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pubmed:articleTitle |
Cytosine deaminase suicide gene therapy for peritoneal carcinomatosis.
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pubmed:affiliation |
Laboratory of Medical Chemistry/Medical Oncology, University of Liège, Belgium.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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