Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2000-3-24
pubmed:abstractText
Leaf metabolites, adenylates, and Rubisco activation were studied in two transgenic tobacco (Nicotiana tabacum L. cv W38) types. Plants with reduced amounts of cytochrome b/f complex (anti-b/f) have impaired electron transport and a low transthylakoid pH gradient that restrict ATP and NADPH synthesis. Plants with reduced glyceraldehyde 3-phosphate dehydrogenase (anti-GAPDH) have a decreased capacity to use ATP and NADPH in carbon assimilation. The activation of the chloroplast NADP-malate dehydrogenase decreased in anti-b/f plants, indicating a low NADPH/NADP(+) ratio. The whole-leaf ATP/ADP in anti-b/f plants was similar to wild type, while it increased in anti-GAPDH plants. In both plant types, the CO(2) assimilation rates decreased with decreasing ribulose 1, 5-bisphosphate concentrations. In anti-b/f plants, CO(2) assimilation was further compromised by reduced carbamylation of Rubisco, whereas in anti-GAPDH plants the carbamylation remained high even at subsaturating ribulose 1,5-bisphosphate concentrations. We propose that the low carbamylation in anti-b/f plants is due to reduced activity of Rubisco activase. The results suggest that light modulation of activase is not directly mediated via the electron transport rate or stromal ATP/ADP, but some other manifestation of the balance between electron transport and the consumption of its products. Possibilities include the transthylakoid pH gradient and the reduction state of the acceptor side of photosystem I and/or the degree of reduction of the thioredoxin pathway.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-10430961, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-11541978, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16593629, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16659671, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16661290, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16661806, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16662500, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16662653, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16663982, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16665524, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16665884, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16666396, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16666614, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16667230, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16667737, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16667747, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16667909, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16667910, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16668101, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-16669043, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-2480892, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-2996524, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-2998220, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-3199, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-3743547, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-3857021, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-7236594, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-7766043, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-8278543, http://linkedlifedata.com/resource/pubmed/commentcorrection/10677442-9414564
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0032-0889
pubmed:author
pubmed:issnType
Print
pubmed:volume
122
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
491-504
pubmed:dateRevised
2010-9-10
pubmed:meshHeading
pubmed:year
2000
pubmed:articleTitle
The role of chloroplast electron transport and metabolites in modulating Rubisco activity in tobacco. Insights from transgenic plants with reduced amounts of cytochrome b/f complex or glyceraldehyde 3-phosphate dehydrogenase.
pubmed:affiliation
Molecular Plant Physiology, Research School of Biological Sciences, The Australian National University, G.P.O. Box 475, Canberra, Australian Capital Territory 2601, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't