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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
7
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pubmed:dateCreated |
2000-3-13
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pubmed:abstractText |
The passive transbilayer movement-flip-flop-was investigated on planar bilayer lipid membranes (BLMs), containing myristic, stearic, or linoleic long-chain fatty acids (FA). In response to a transbilayer pH gradient, a difference in the surface charges between inner and outer leaflets appeared. Because the BLM was formed from FA and neutral lipid, a surface potential difference was originated solely by a concentration difference of the initially equally distributed ionized FA. As revealed by zeta-potential measurements, the corresponding surface potential difference DeltaPhi(s) was at least twice the value expected from a titration of the FA alone. The additional surface charge was attributed to FA flip-flop induced by the transbilayer pH gradient. DeltaPhi(s) was derived from capacitive current measurements carried out with a direct current (dc) bias and was corrected for changes of membrane dipole potential Phi(d). Dual-wavelength ratiometric fluorescence measurements have shown that Phi(d) values of the pure DPhPC bilayers and BLMs containing 40 mol % FA differ by less than 6%. It is concluded that fast FA flip-flop is not restricted to membranes with high curvature. The role of pH gradient as an effective driving force for the regulation of FA uptake is discussed.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(3-sulfonatopropyl)-4-(beta)(2-(di...,
http://linkedlifedata.com/resource/pubmed/chemical/Buffers,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids, Nonesterified,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Lipid Bilayers,
http://linkedlifedata.com/resource/pubmed/chemical/Liposomes,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylethanolamines,
http://linkedlifedata.com/resource/pubmed/chemical/Pyridinium Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Stearic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/diphytanoylphosphatidylethanolamine,
http://linkedlifedata.com/resource/pubmed/chemical/stearic acid
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-2960
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1834-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10677234-Buffers,
pubmed-meshheading:10677234-Fatty Acids,
pubmed-meshheading:10677234-Fatty Acids, Nonesterified,
pubmed-meshheading:10677234-Fluorescent Dyes,
pubmed-meshheading:10677234-Hydrogen-Ion Concentration,
pubmed-meshheading:10677234-Lipid Bilayers,
pubmed-meshheading:10677234-Liposomes,
pubmed-meshheading:10677234-Membrane Potentials,
pubmed-meshheading:10677234-Phosphatidylethanolamines,
pubmed-meshheading:10677234-Potentiometry,
pubmed-meshheading:10677234-Pyridinium Compounds,
pubmed-meshheading:10677234-Spectrometry, Fluorescence,
pubmed-meshheading:10677234-Stearic Acids,
pubmed-meshheading:10677234-Surface Properties
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pubmed:year |
2000
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pubmed:articleTitle |
Changes of intrinsic membrane potentials induced by flip-flop of long-chain fatty acids.
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pubmed:affiliation |
Department of Medical Physics and Biophysics, Martin-Luther-University, 06097 Halle/Saale, Germany. elena.pohl@medizin.uni-halle.de
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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