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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-2-24
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pubmed:databankReference |
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pubmed:abstractText |
The structure of a Nova protein K homology (KH) domain recognizing single-stranded RNA has been determined at 2.4 A resolution. Mammalian Nova antigens (1 and 2) constitute an important family of regulators of RNA metabolism in neurons, first identified using sera from cancer patients with the autoimmune disorder paraneoplastic opsoclonus-myoclonus ataxia (POMA). The structure of the third KH domain (KH3) of Nova-2 bound to a stem loop RNA resembles a molecular vise, with 5'-Ura-Cyt-Ade-Cyt-3' pinioned between an invariant Gly-X-X-Gly motif and the variable loop. Tetranucleotide recognition is supported by an aliphatic alpha helix/beta sheet RNA-binding platform, which mimics 5'-Ura-Gua-3' by making Watson-Crick-like hydrogen bonds with 5'-Cyt-Ade-3'. Sequence conservation suggests that fragile X mental retardation results from perturbation of RNA binding by the FMR1 protein.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
4
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pubmed:volume |
100
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
323-32
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pubmed:dateRevised |
2010-9-15
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pubmed:meshHeading |
pubmed-meshheading:10676814-Amino Acid Motifs,
pubmed-meshheading:10676814-Amino Acid Sequence,
pubmed-meshheading:10676814-Antigens, Neoplasm,
pubmed-meshheading:10676814-Autoantigens,
pubmed-meshheading:10676814-Binding Sites,
pubmed-meshheading:10676814-Crystallography, X-Ray,
pubmed-meshheading:10676814-Fragile X Syndrome,
pubmed-meshheading:10676814-Models, Molecular,
pubmed-meshheading:10676814-Molecular Sequence Data,
pubmed-meshheading:10676814-Nerve Tissue Proteins,
pubmed-meshheading:10676814-Paraneoplastic Syndromes, Nervous System,
pubmed-meshheading:10676814-Protein Structure, Tertiary,
pubmed-meshheading:10676814-RNA-Binding Proteins,
pubmed-meshheading:10676814-Ribonucleoproteins,
pubmed-meshheading:10676814-Sequence Homology, Amino Acid
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pubmed:year |
2000
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pubmed:articleTitle |
Sequence-specific RNA binding by a Nova KH domain: implications for paraneoplastic disease and the fragile X syndrome.
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pubmed:affiliation |
Laboratories of Molecular Biophysics, The Rockefeller University, New York, New York 10021, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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