Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2000-4-3
pubmed:databankReference
pubmed:abstractText
We isolated a mutation (rlr1-1; required for lacZ RNA) in the Saccharomyces cerevisiae (Sc) RLR1 gene as a suppressor of sin4, a component of the Mediator subcomplex of the RNA polymerase II holoenzyme and a determinant of chromatin structure. RLR1 encodes a deduced protein found also in fission yeast, nematode worms, and humans. The presence of these orthologs suggests that Rlr1 family members comprise a class of putative KEKE motif-containing proteins, characteristic of certain chaperones as well as regulators and subunits of the mammalian 20S proteasome. A role for RLR1 (THO2) in transcription appears to occur at a step subsequent to transcription initiation (see also Piruat, J.I. and Aguilera, A., 1998. EMBO J. 17, 4859-4872); Sc genes fused to the reporter gene lacZ were expressed at a very low level, while the corresponding native chromosomal genes were expressed at approximately normal levels in rlr1 mutants. Our studies show that rlr1 mutations cause a wide range of growth defects in addition to their novel affect on lacZ.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/CYC1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome c Group, http://linkedlifedata.com/resource/pubmed/chemical/Cytochromes c, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal, http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Mediator Complex, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/SIN4 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
243
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
195-205
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10675628-Amino Acid Sequence, pubmed-meshheading:10675628-Cell Division, pubmed-meshheading:10675628-Cloning, Molecular, pubmed-meshheading:10675628-Conserved Sequence, pubmed-meshheading:10675628-Cytochrome c Group, pubmed-meshheading:10675628-Cytochromes c, pubmed-meshheading:10675628-DNA, Fungal, pubmed-meshheading:10675628-Fungal Proteins, pubmed-meshheading:10675628-Gene Expression Regulation, Fungal, pubmed-meshheading:10675628-Humans, pubmed-meshheading:10675628-Lac Operon, pubmed-meshheading:10675628-Mediator Complex, pubmed-meshheading:10675628-Molecular Sequence Data, pubmed-meshheading:10675628-Mutation, pubmed-meshheading:10675628-Phylogeny, pubmed-meshheading:10675628-Recombinant Fusion Proteins, pubmed-meshheading:10675628-Saccharomyces cerevisiae, pubmed-meshheading:10675628-Saccharomyces cerevisiae Proteins, pubmed-meshheading:10675628-Sequence Alignment, pubmed-meshheading:10675628-Sequence Analysis, DNA, pubmed-meshheading:10675628-Sequence Homology, Amino Acid, pubmed-meshheading:10675628-Spores, Fungal, pubmed-meshheading:10675628-Trans-Activators, pubmed-meshheading:10675628-Transcription Factors
pubmed:year
2000
pubmed:articleTitle
RLR1 (THO2), required for expressing lacZ fusions in yeast, is conserved from yeast to humans and is a suppressor of SIN4.
pubmed:affiliation
Department of Biochemistry, State University of New York, Health Science Center at Syracuse, Syracuse, NY, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't