Linked Life Data

10675566

Source:http://linkedlifedata.com/resource/pubmed/id/10675566

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2-3
pubmed:dateCreated
2000-3-31
pubmed:abstractText
The data presented implicate a GATA binding site in the transcriptional regulation of 15-lipoxygenase-1 (15-LO-1) gene expression in human colorectal carcinoma Caco-2 cells. High expression of GATA-6 mRNA and protein was observed, while GATA-4 mRNA was expressed at a very low level in Caco-2 cells. The expression of GATA-6 was down-regulated, while 15-LO-1 expression was dramatically up-regulated after treatment with sodium butyrate (NaBT). A study using an electrophoretic mobility shift assay indicated that a GATA binding site of the 15-LO-1 promoter region binds to GATA proteins present in both undifferentiated and, to a lesser extent, NaBT-treated (differentiated) Caco-2 cells. Moreover, that DNA binding shift band was disrupted after the addition of GATA-6 antibody in a supershift assay in the absence of NaBT, suggesting that GATA-6 is bound to the GATA binding site of the 15-LO-1 promoter in undifferentiated cells. In contrast, the addition of GATA-6 antibody did not affect the DNA binding ability in NaBT-induced differentiated cells. On the other hand, mutation of the GATA site of the 15-LO-1 promoter decreased the transactivation of the 15-LO-1 promoter as measured by luciferase activity in both FBS and NaBT cultured cells, indicating an unknown GATA binding protein to up-regulate 15-LO-1 expression. These implicate the GATA site at -240 of the proximal region of the 15-LO-1 promoter in the basic transcription of 15-LO-1 gene expression in Caco-2 cells, with GATA-6 acting to repress 15-LO-1 expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0014-5793
pubmed:author
pubmed:issnType
Print
pubmed:day
11
pubmed:volume
467
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
341-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10675566-Arachidonate 15-Lipoxygenase, pubmed-meshheading:10675566-Binding Sites, pubmed-meshheading:10675566-Blotting, Western, pubmed-meshheading:10675566-Caco-2 Cells, pubmed-meshheading:10675566-Carcinoma, pubmed-meshheading:10675566-Cell Differentiation, pubmed-meshheading:10675566-Colorectal Neoplasms, pubmed-meshheading:10675566-DNA-Binding Proteins, pubmed-meshheading:10675566-Erythroid-Specific DNA-Binding Factors, pubmed-meshheading:10675566-GATA4 Transcription Factor, pubmed-meshheading:10675566-GATA6 Transcription Factor, pubmed-meshheading:10675566-Gene Expression Regulation, pubmed-meshheading:10675566-Humans, pubmed-meshheading:10675566-Promoter Regions, Genetic, pubmed-meshheading:10675566-RNA, Messenger, pubmed-meshheading:10675566-Transcription Factors
pubmed:year
2000
pubmed:articleTitle
A GATA binding site is involved in the regulation of 15-lipoxygenase-1 expression in human colorectal carcinoma cell line, caco-2.
pubmed:affiliation
Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Sciences, PO Box 12233, Research Triangle Park, NC, USA.
pubmed:publicationType
Journal Article