Source:http://linkedlifedata.com/resource/pubmed/id/10675409
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 3
|
| pubmed:dateCreated |
2000-3-27
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| pubmed:abstractText |
A canine isolate (strain T1) of simian virus 5 (SV-5) performed multiple replication in BHK cells but did not induce cell fusion for up to 3 days. In contrast, a prototype strain (WR) provoked extensive cell fusion within 2 days during the course of its replication. Accordingly, the fusion (F) protein of the T1 strain did not cause cell fusion even when co-expressed with the SV-5 haemagglutinin-neuraminidase (HN) protein, whereas the WR F protein induced cell fusion in the presence of the HN protein. Differences in the predicted amino acid sequences of the T1 and WR F proteins were found at 12 positions and it was proved that the T1 F protein had a longer cytoplasmic tail than the WR F protein. By reducing the length of the cytoplasmic tail or by replacing the tail with the WR F counterpart, the T1 F protein partly restored its HN-dependent fusing activity. Chimeric and mutational analyses between the T1 F protein and the mutant F protein (L22P) suggested that Glu-132 in the heptad repeat 1 domain was involved in the HN-independent fusing activity in addition to the previously identified Pro-22 at the F(2) N terminus. It was also shown that Ala-290 in the heptad repeat 3 domain contributed to the HN-independent fusing activity to some extent.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
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| pubmed:issn |
0022-1317
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
81
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
719-27
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10675409-Amino Acid Sequence,
pubmed-meshheading:10675409-Animals,
pubmed-meshheading:10675409-Cell Fusion,
pubmed-meshheading:10675409-Cell Line,
pubmed-meshheading:10675409-Cercopithecus aethiops,
pubmed-meshheading:10675409-Cricetinae,
pubmed-meshheading:10675409-Dogs,
pubmed-meshheading:10675409-Hemagglutinins, Viral,
pubmed-meshheading:10675409-Molecular Sequence Data,
pubmed-meshheading:10675409-Mutagenesis, Site-Directed,
pubmed-meshheading:10675409-Neuraminidase,
pubmed-meshheading:10675409-Protein Structure, Tertiary,
pubmed-meshheading:10675409-Recombinant Fusion Proteins,
pubmed-meshheading:10675409-Respirovirus,
pubmed-meshheading:10675409-Sequence Homology, Amino Acid,
pubmed-meshheading:10675409-Vero Cells,
pubmed-meshheading:10675409-Viral Fusion Proteins,
pubmed-meshheading:10675409-Virus Replication
|
| pubmed:year |
2000
|
| pubmed:articleTitle |
An amino acid in the heptad repeat 1 domain is important for the haemagglutinin-neuraminidase-independent fusing activity of simian virus 5 fusion protein.
|
| pubmed:affiliation |
Department of Microbiology, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|