Linked Life Data

10674215

Source:http://linkedlifedata.com/resource/pubmed/id/10674215

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-3-9
pubmed:abstractText
Potato plants (Solanum tuberosum L., cv. Désirée) were transformed with the polyphosphate kinase gene from Escherichia coli fused to the leader sequence of the ferredoxin oxidoreductase gene (FNR) from Spinacea oleracea under the control of the leaf specific St-LS1 promoter to introduce a novel phosphate pool in the chloroplasts of green tissues. Transgenic plants (cpPPK) in tissue culture developed necrotic lesions in older leaves and showed earlier leaf senescence while greenhouse plants showed no noticeable phenotype. Leaves of cpPPK plants contained less starch but higher concentrations of soluble sugars. The presence of polyphosphate in cpPPK leaves was demonstrated by toluidine blue staining and unambiguously verified and quantified by in vitro 31P-NMR of extracts. Polyphosphate accumulated during leaf development from 0.06 in juvenile leaves to 0.83 mg P g-1 DW in old leaves and had an average chain length of 18 residues in mature leaves. In situ 31P-NMR on small leaf pieces perfused with well-oxygenated medium showed only 0.036 mg P g-1 DW polyphosphate that was, however, greatly increased upon treatment with 50 mM ammonium sulfate at pH 7.3. This phenomenon along with a yield of 0.47 mg P g-1 DW polyphosphate from an extract of the same leaf material suggests that 93% of the polyphosphate pool is immobile. This conclusion is substantiated by the observation that no differences in polyphosphate pool sizes could be discerned between darkened and illuminated leaves, leaves treated with methylviologen or anaerobis and control leaves, treatments causing a change in the pool of ATP available for polyPi synthesis. Results are discussed in the context of the chelating properties of polyphosphates for cations and its consequences for the partitioning of photoassimilate between starch and soluble sugars.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0168-1656
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
65-80
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10674215-Animals, pubmed-meshheading:10674215-Carbohydrate Metabolism, pubmed-meshheading:10674215-Cell Compartmentation, pubmed-meshheading:10674215-Chloroplasts, pubmed-meshheading:10674215-Coloring Agents, pubmed-meshheading:10674215-Ethanol, pubmed-meshheading:10674215-Magnetic Resonance Spectroscopy, pubmed-meshheading:10674215-Phosphorus Isotopes, pubmed-meshheading:10674215-Phosphotransferases (Phosphate Group Acceptor), pubmed-meshheading:10674215-Plant Leaves, pubmed-meshheading:10674215-Plant Proteins, pubmed-meshheading:10674215-Plants, Genetically Modified, pubmed-meshheading:10674215-Polyphosphates, pubmed-meshheading:10674215-Recombinant Fusion Proteins, pubmed-meshheading:10674215-Solanum tuberosum, pubmed-meshheading:10674215-Solvents, pubmed-meshheading:10674215-Tolonium Chloride, pubmed-meshheading:10674215-Transgenes
pubmed:year
2000
pubmed:articleTitle
Introduction of polyphosphate as a novel phosphate pool in the chloroplast of transgenic potato plants modifies carbohydrate partitioning.
pubmed:affiliation
Department of Plant Sciences, Wageningen University, The Netherlands.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't