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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2000-3-17
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pubmed:abstractText |
Phosphorylation of transcription factors is a key link between cell signaling and the control of gene expression. Here we report that phosphorylation regulates DNA binding of the Ets-1 transcription factor by reinforcing an autoinhibitory mechanism. Quantitative DNA-binding assays show that calcium-dependent phosphorylation inhibits Ets-1 DNA binding 50-fold. The four serines that mediate this inhibitory effect are distant from the DNA-binding domain but near structural elements required for autoinhibition. Mutational analyses demonstrate that an intact inhibitory module is required for phosphorylation-dependent regulation. Partial proteolysis studies indicate that phosphorylation stabilizes an inhibitory conformation. These findings provide a structural mechanism for phosphorylation-dependent inhibition of Ets-1 DNA binding and demonstrate a new function for inhibitory modules as structural mediators of negative signaling events.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-10075931,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-10075932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-10212261,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-10212281,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-10384327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-10594011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-10594012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-1311254,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-1339307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-1351401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-1409581,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-1589769,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-1592263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-1592264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-1779832,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-1900874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-2187191,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-2404972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-2573431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-2610349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-3136014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-7566176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-7566177,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-7569926,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-7935472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-7958933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-7961750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8003962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8193547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8264651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8386592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8552081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8557686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8598195,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8602247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8628272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8654922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8745408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8783562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-8824592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-9094628,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-9143705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-9171356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-9190210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-9190901,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-9242925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-9305854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-9460239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-9461436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-9501094,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10673508-9806641
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Calmodulin-Dependent...,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Extracts,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/ETS1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoserine,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Protein c-ets-1,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-ets,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0890-9369
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
14
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
366-76
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10673508-Humans,
pubmed-meshheading:10673508-Phosphorylation,
pubmed-meshheading:10673508-DNA,
pubmed-meshheading:10673508-Amino Acid Sequence,
pubmed-meshheading:10673508-Models, Biological,
pubmed-meshheading:10673508-Molecular Sequence Data,
pubmed-meshheading:10673508-Protein Structure, Tertiary,
pubmed-meshheading:10673508-T-Lymphocytes,
pubmed-meshheading:10673508-Cell Extracts,
pubmed-meshheading:10673508-DNA-Binding Proteins,
pubmed-meshheading:10673508-Transcription Factors,
pubmed-meshheading:10673508-Phosphoserine,
pubmed-meshheading:10673508-Calcium-Calmodulin-Dependent Protein Kinases
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